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You have carried out an experiment using the spectrophotometry concept. A solution containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a blue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM: 1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).

You have carried out an experiment using the spectrophotometry concept. A solution containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a blue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM: 1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You have carried out an experiment using the spectrophotometry concept. A solution
containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a
blue colour whose absorbance (A) could be read at 550 nm. The results are shown below.

If the standard solution (compound X) used have a concentration of 1 mM:
1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).

Test tube no. 2 3 4 5 6 7 Sample Y Standard solution X (ml) 0 0.1 0.2 0.4 0.6 0.8 1.0 1.0 Reagent 1 Reagent 2 (ml) (ml) 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 Water (ml) 1.0 0.9 0.8 0.6 0.4 0.2 0.0 0.0 A550 nm 0.0 0.05 0.12 0.24 0.35 0.48 0.59 0.35
Transcribed Image Text:Test tube no. 2 3 4 5 6 7 Sample Y Standard solution X (ml) 0 0.1 0.2 0.4 0.6 0.8 1.0 1.0 Reagent 1 Reagent 2 (ml) (ml) 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 0.3 3.0 Water (ml) 1.0 0.9 0.8 0.6 0.4 0.2 0.0 0.0 A550 nm 0.0 0.05 0.12 0.24 0.35 0.48 0.59 0.35
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