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(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table I was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 0 0.025 0.05 0,075 0.1 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table I estimate the amino acid concentration of test sample A. (i) (ii) I 2 3 Absorbance @575nm 4 5 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimental data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table I was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Tube Number Standard (mM)/Test sample A 0 0.025 0.05 0,075 0.1 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table I estimate the amino acid concentration of test sample A. (i) (ii) I 2 3 Absorbance @575nm 4 5 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimental data. (iii) Describe the principle of the Ninhydrin method for quantitation of amino acids.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table I was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Absorbance @575nm Tube Number Standard (mM)/Test sample A 0 0.025 0.05 0,075 0.1 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table I estimate the amino acid concentration of test sample A. (i) (ii) (iii) 1 2 3 4 5 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimental data. Describe the principle of the Ninhydrin method for quantitation of amino acids.
Transcribed Image Text:(a) In an experiment to determine the glycine concentration of test sample A, the Ninhydrin method was used and the absorbance data in Table I was generated. Table 1: Absorbance data generated @ Amax for glycine standards (0-0.1mM) and test sample A. Absorbance @575nm Tube Number Standard (mM)/Test sample A 0 0.025 0.05 0,075 0.1 Test sample A (undiluted) Test sample A (2-fold dilution) Test sample A (5-fold dilution) Using the data in Table I estimate the amino acid concentration of test sample A. (i) (ii) (iii) 1 2 3 4 5 6 7 8 0 0.126 0.231 0.355 0.479 0.723 0.357 0.143 Given the true value of test sample A is 0.15mM, comment on the accuracy and precision of the experimental data. Describe the principle of the Ninhydrin method for quantitation of amino acids.
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