You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the the following procedures? Answer briefly but completely. purpose of Using sodium dodecyl sulfate, a detergent Answer: a. b. Adding RNase A and Proteinase K during extraction Answer: с. Adding ethanol before recovering the DNA extract Answer:
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
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#3 Mol Bio Gene o...
Q
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Complete the following tasks.
You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an
enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.
Task 1: DNA Extraction
To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of
the following procedures? Answer briefly but completely.
Using sodium dodecyl sulfate, a detergent
Answer:
а.
b. Adding RNase A and Proteinase K during extraction
Answer:
Adding ethanol before recovering the DNA extract
с.
Answer:
Task 2: Polymerase Chain Reaction
After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR
using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer
briefly but completely.
DNA polymerase isolated from Thermus aquaticus
a.
Answer:
b. Deoxynucleotide triphosphates (DNTPS)
Answer:
Forward and reverse primers
с.
Answer:
Task 3: Agarose Gel Electrophoresis
Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded
with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D)
PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane
is which. You are shown a diagram of the obtained gel below.
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