Why is sequencing an essential part if generating mutant protein? What could happen if you didn’t sequence, but rather went directly from mini-prep to transforming NEB express I^q cells?
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Why is sequencing an essential part if generating mutant protein? What could happen if you didn’t sequence, but rather went directly from mini-prep to transforming NEB express I^q cells?
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- In the regulatory switch experiment, what do you predict is a likely outcome in mice with the bat-mouse construct? Select one 1.) change in the nucleotide sequence of the Prx1 transcribed region 2.) change in the amount of Prx1 mRNA produced 3.)change in the amino acid sequence of the PRX1 protein 4.)change in the nucleotide sequence of the Prx1 mRNA producedHow can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted into a genome?Why is it important that NEB express I^q cells do not have endonuclease? What could happen if they did?
- In the experiment summarized below, scientists were examining the presence of specific sequences in individuals with age. In this experiment they extracted DNA from lymphocytes of various aged individuals and measured the length of a TTAGGG (in kb) repeat they found in their genomic DNA (Left Panel). In the right panel, the scientists measured the length of the same repeats in individuals with lymphocyte failure (red dots most severely effected) that have a mutation in a critical enzyme. Answer the following questions in 2-3 sentences each. A. What is the name of the specific sequence the scientists are measuring in the experiment shown below. B. For the individuals with lymphocyte pathology in the right panel, which gene is likely defective that causes the data shown? C. Explain why the length of the repeat sequence decreases with age.How can the efficiency of homologous recombination be improved when using CRISPR-Cas9, compared to the traditional ES cell homologous recombination method?What are site-recombinases? Describe in detail how cre- recombinase can be used to decipher the roles of specific genes and proteins in complex multicellular organisms? Explain how the cre-lox system can be used to examine the role of a particular gene in a specific type of tissue?
- Q1: What common mechanism is employed by the guide RNA to find its target DNA sequence? Q2: How many strands of DNA must Cas9 cut to be effective? Q3: Does Cas9 also cause the deletion of DNA from the genome?If want to express the gene from monkey, the PET vector or BACMAN would be best? Briefly explainWhat is Taq polymerase and why is it important?
- When researchers cloned the ey, Pax-6, and AN genes, What did they found?You are using Drosophila as a model system to investigate eye development. You do positional cloning to isolate a mutant gene called eyeless (eyl), which causes flies to develop with reduced sized eyes when homozygous. Using several DNA markers from the candidate interval as probes for colony hybridization to a CDNA library, you obtain a 10 Kb cDNA insert cloned into the unique EcoRI site of a 3.5 Kb plasmid vector. a. Briefly describe how would you use the Drosophila eyl cDNA clone to identify a clone from a mouse genomic DNA library that is homologous to the Drosophila eyl gene? b. You isolate a clone of mouse genomic DNA that appears to be homologous to the Drosophila eyl gene. After DNA sequencing of a small piece of the mouse genomic clone, you use the DNA sequence to search the mouse genome database. You find that there is a SNP locus (n1234) that does NOT cause an RFLP in this genomic clone. You have identified several breeding lines of mice, each having been bred for several…i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion protein. Please describe the strategy that you will follow to edit this gene with CRISPR-Cas9 technology, in order to visualize the expression of gene X in live cells. (ii) Please explain how a knockout mouse for a gene X can be produced with CRISPR-Cas9 technology