Which of the following is the most likely result of a mutation in the lac operator that prevents release of the depressor protein? Choose 1 answer: (A)The lactose permease protein will be produced, even in the absence of lactose. (B) Transcription of the structural genes will be blocked, even in the presence of lactose. (C) The catabolite activator protein will be bound to the CAP site, even in the presence of glucose. (D) Expression of the lacI gene will be repressed, even in the absence of glucose.

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Which of the following is the most likely result of a mutation in the lac operator that prevents release of the depressor protein? Choose 1 answer: (A)The lactose permease protein will be produced, even in the absence of lactose. (B) Transcription of the structural genes will be blocked, even in the presence of lactose. (C) The catabolite activator protein will be bound to the CAP site, even in the presence of glucose. (D) Expression of the lacI gene will be repressed, even in the absence of glucose.
The lac operon in E. coli is a coordinately controlled set of genes required for lactose metabolism. The
operon and its associated regulatory sequences are shown in the following diagram.
Structural genes
Promoter lacl
CAP site Promoter Operator
lacZ
lacY
lacA
The function of each locus shown in the diagram is listed in the table below.
Part
Function
Promoter
Binding site for RNA polymerase
lacl
Gene encoding the lac repressor protein
CAP site
Binding site for the catabolite activator protein
Operator
Binding site for the lac repressor protein
lacZ
Gene encoding the beta-galactosidase protein
lacY
Gene encoding the lactose permease protein
lacA
Gene encoding the galactoside transacetylase protein
The activity of the lac operon is influenced by the availability of lactose and glucose in the cellular
environment. In the presence of lactose, the lac repressor protein is released from the operator, which
allows the structural genes to be transcribed at a low level. If glucose (the preferred carbon source) is
absent during this time, the catabolite activator protein binds to the CAP site, causing the structural genes
to be transcribed at a higher level.
Transcribed Image Text:The lac operon in E. coli is a coordinately controlled set of genes required for lactose metabolism. The operon and its associated regulatory sequences are shown in the following diagram. Structural genes Promoter lacl CAP site Promoter Operator lacZ lacY lacA The function of each locus shown in the diagram is listed in the table below. Part Function Promoter Binding site for RNA polymerase lacl Gene encoding the lac repressor protein CAP site Binding site for the catabolite activator protein Operator Binding site for the lac repressor protein lacZ Gene encoding the beta-galactosidase protein lacY Gene encoding the lactose permease protein lacA Gene encoding the galactoside transacetylase protein The activity of the lac operon is influenced by the availability of lactose and glucose in the cellular environment. In the presence of lactose, the lac repressor protein is released from the operator, which allows the structural genes to be transcribed at a low level. If glucose (the preferred carbon source) is absent during this time, the catabolite activator protein binds to the CAP site, causing the structural genes to be transcribed at a higher level.
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