When pure reduced cytochrome c is added to carefully preparedmitochondria along with ADP, P, antimycin A, and oxygen, thecytochrome c becomes oxidized, and ATP is formed, with a P/oratio approaching 1.0.(a) Indicate the probable flow of electrons in this system.(b) Why was antimycin A added?(c) What does this experiment tell you about the location of cou-pling sites for oxidative phosphorylation?(d) Write a balanced equation for the overall reaction (including cytc oxidation and ATP synthesis).

Cytochrome c is a hemeprotein and is a vital component of electron transport chain. The transfer of…

Answered: When pure reduced cytochrome c is added…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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14) Which of the following statements is true under the conditions provided: the enzyme concentration is 0.5 nM, substrate concentration is 10 µM, and the KM = 20 µM? a) The enzymatic reaction occurs at maximal velocity. b) The enzymatic reaction occurs between half-maximal and maximal velocity. c) The enzymatic reaction occurs at around half-maximal velocity. d) The enzymatic reaction occurs at between zero and half-maximal velocity. e) Not enough information given to know about the enzymatic reaction rate.
(A) What is the free energy yield associated with the transport of a proton from the mitochondrial intermembrane space (IMS) to the mitochondrial matrix, assuming that the pH of the IMS is 1.4 units lower than the pH of the matrix? (Hint: Calculate AGO' for the reaction H"IMS > H matris using the relationship AG° = RTInKeg and remembering that pH = -log1o[H*]. Assume that T = 310K (37°C), and R = 8.315x10-3 kJ mol-1 K-1. Show your work and include correct units!)
It is estimated that each electron pair donated by NADH leads to the synthesis of approximately three ATP molecules, whereas each electron pair donated by FADH2 leads to the synthesis of approximately two ATP molecules. What is the underlying reason for the difference in yield for electrons donated by FADH2 versus NADH?
Yeast produces carbon dioxide gas. Therefore, based only on this information, can we tell whether the yeast were using aerobic cellular respiration or Anaerobic (ethanol) fermentation? Yes or No If not, what additional information would we need to know to tell whether the yeast used aerobic respiration or anaerobic (Ethanol) fermentation?
1. Cyanide, oligomycin, and 2,4-dinitrophenol are all inhibitors of oxidative phosphorylation in mitochon- dria. Provide an explanation to the following conditions regarding these potent inhibitors. (a) Explain why adding cyanide to an active in vitro suspension of mitochondria blocks ATP synthesis. What happens to the rate of ATP synthesis when 2,4-dinitrophenol is added to this mitochon- drial suspension after it was treated with cyanide? (b) Explain why the rate of oxygen consumption decreases in an in vitro suspension of mito- chondria when oligomycin is added. What happens to the rate of oxygen consumption in this oligomycin- inhibited system after adding 2,4-dinitrophenol? Explain.
In terms of energy transduction (a) in what way are mitochondrial complexes I, II, and IV similar? And (b) in what way are they different?
Citrate and isocitrate are interconverted via the enzyme aconitase: The standard free energy change for this reaction is +6.7 kJ/mol. In my pet llama's mitochondria, the free energy change was measured to be +0.8 kJ/mol. (No llamas were harmed in this experiment!) a. Calculate the [isocitrate]/[citrate] ratio in these mitochondria. b. If [isocitrate] =0.03 mM, what is [citrate]?
What compounds are the immediate source of the "high energy electrons" that enter the electron transport system of mitochondria? Where do these electron-donating compounds gain their electrons? What type of compounds are these electron-donating compounds?
Calculate how many moles of ATP can be made in standard conditions when 1 mole of protons flow back into the mitochondrial matrix through the ATP synthase. Assume that: i) the matrix pH is 7.8, ii) the cytosolic pH is 7.2, iii)ΔE=0.168 V (inside negative), iv) T=37°C, and v) the standard free energy change for the hydrolysis of 1 mole of ATP is -31.3 kJ/mol.
Give only typing answer with explanation and conclusion
Which of the following is NOT true of Complex 1? a.) it's a proton pump and it pumps 4H+ into the intermembrane space for every pair of electrons donated by NADH. b.) electrons from NADH are accepted by an FMN prosthetic group within Complex I. c.) electrons from NADH are passed through Complex 1 prosthetic groups to ubiquinone. d.) it accepts electrons from either NADH or FADH2
In the early days of “mitochondriology,” P/O ratios were determined from measurements of volume of O2 taken up by respiring mitochondria and chemical assays for disappearance of inorganic phosphate. Now, however, it is possible to measure P/O ratios simply with a recording oxygen electrode. How might this be done?

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