What would be the order of elution when using a capillary electrophoresis to separate the following ions: K+, Zn2+, Se2–? In this separation, the separation was performed in the normal mode where the cathode (–) is near the detector and the anode (+) is where the sample is applied. Group of answer choices K+; Zn2+; Se2- Zn2+; K+; Se2- Se2-; K+; Zn2+ Se2-; Zn2+; K+ Zn2+; Se2-; K+ K+; Se2-; Zn2+
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- I U X2 More Formatting A A Q 4. A fluoride ion selective electrode was used to analyse for fluoride as part of the QC procedures during the production of Listerine Fluoride Defense Anticavity Mouthwash. The mouthwash contains 0.02% sodium fluoride which is equivalent to 90 ppm of fluoride. The QC specification for the mouthwash is 90 ± 5 ppm of fluoride. During production three standards and six periodic samples were analysed each hour. The mouthwash samples were diluted 100 fold before the pH and ionic strength were adjusted using an appropriate TISAB before analysis. The following data was obtained for the standards and the calibration curve is shown below. (i) (ii) Solution E (MV) 1.00 x 104 MF 40.5 5.00 x 10-5 MF 1.00 x 10-5 M F 60.2 100.0 Six samples were analysed and the following potentials, measured in mV, were obtained; 58.9, 61.0, 60.7, 59.3, 59.6 and 61.7. Use this data to determine the fluoride concentration in the mouthwash in ppm (RAM fluoride 19.0). Comment on whether…A method for the analysis of Ca2+ in water suffers from an interference in the presence of Zn2+. When the concentration of Ca2+ is 100 times greater than that of Zn2+, an analysis for Ca2+ gives a relative error of +0.5%. What is the selectivity coefficient for this method?E (MV) (b) A fluoride ion selective electrode was used to analyse for fluoride as part of the QC procedures during the production of Listerine Fluoride Defense Anticavity Mouthwash. The mouthwash contains 0.02% sodium fluoride which is equivalent to 90 ppm of fluoride. The QC specification for the mouthwash is 90 ± 5 ppm of fluoride. During production three standards and six periodic samples were analysed each hour. The mouthwash samples were diluted 100 fold before the pH and ionic strength were adjusted using an appropriate TISAB before analysis. The following data was obtained for the standards and the calibration curve is shown below. Table 1. Solution E (MV) 1.00 x 10 M F 5.00 x 10-5 M F 40.5 60.2 1.00 x 10-5 M F 100.0 Figure 5. Continued... 120 -100 -80 -60 -40 -20 +o -3.5 -3.7 -3.9 -4.1 -4.3 -4.5 -4.7 -4.9 -5.1 -5.3 -5.5 Log [fluoride] (i) Six samples were analysed and the following potentials, measured in mV, were obtained; 58.9, 61.0, 60.7, 59.3, 59.6 and 61.7. Use this data…
- The potassium ion selective electrode uses valinomycin as its ionophore to achieve high level of selectivity over other ions. Given the following selectivity data (kij) for this ISE, answer the following questions: (You may assume that activity = concentration). Interferant ion Na+ Ca²+ Mg2+ Log kij -4 -7 -7 a) How much greater must [Na+] be than [K+] for the two ions to give an equal response? (c) If the calibration constant (Econst) was previously found to be 0.197 V. Calculate [K+] if the ISE gives a reading of -0.029 V in a solution which also contains 0.5 M Nat and 0.2 M Ca²+. Give the answer in μM (micro moles per litre).A dye has a molar absorptivity of 2.00 × 103 M–1 cm–1 at 590 nm. What is the %T for an 8.00 × 10–5 M dye solution in a 1.00 cm cell? Question 20 options: 69% 31% 16%TLC was used to compare the solubility of two compounds A and B in a solvent system (acetic acid, methanol, H2O). The distances that solvent has moved in the silica gel TLC was 12 cm. and for the compounds were 2 and 8 cm respectively. (a) Calculate the Rf values and which compound is more soluble and which one is more polar. (b) What are the factors affecting the Rf value
- please show work[3] The following Figure is the electrocapillary curves (A) and differential capacitance curves (B) for mercury in contact with 0.5 M Na,SO4 in the presence and absence of n-heptanol . 440- (A) (B) 420 Na,So. 400 Na, So, CHOH 360 Na so, 20 340- Na So, CH,OH 10 320 0.4 0.8 12 1.6 20 24 0.4 0.8 1.2 -EV ex NCE) 1.6 2.0 -EV e NCE (a) How do the curves in figure (A) relate to those in figure (B)? (b) What implications can be derived from the flat region in the electrocapillary curves in the presence of n-heptyl alcohol? (c) If we measure the electrocapillary curve of mercury in contact with 0.5 M Na,SO4 at higher temperature, what changes in the curve can be observed? Try to give explanation of these changes.Consider the following setup of a capillary electrophoresis (CE) system: What are the two major factors contributing to the moving speed of molecules in a CE separation process? Does the analyte need to be charged for CE separation? Which type of analytes will reach the detector first, positively-charged or negatively-charged?
- A moving boundary experiment was performed at 25°C with 0.100 M KCI on the bottom and (initially) 0.0700 M NaCl on top. The transport numbers of K+ and Na+ in these solutions are 0.490 and 0.388, respectively. The boundary moved downward. The cross-sectional area of the tube was 0.100 cm². The charge passed through was 96.5 coulombs. How far did the boundary move in cm?500 ml of wastewater sample was collected and 200 ml of this sample was analyzed by classical 5 day BOD test (at 20\deg C) with 1/15 dilution. The initial DO measurement result was 9 mg/L and 35% reduction in DO was observed at the end of the experiment. The BOD reaction rate constant was 0.24 d¹ (base e) and there was no change in the DO concentration of the blank. If this wastewater contains 2.35 mg/L ammonia, 0.073 mM nitrate, 0.66 mg/L. nitrite and 7.81 mg/L TKN, answer the following questions:a) What are the organic nitrogen and total nitrogen concentrations in this sample?b) Calculate the BOD, at 10\deg C.(a 4 shows scanning electron microscope (SEM) images of extruded actions of packing bed for two capillary columns of different diameters, al 750 (bottom image) and b) 30-μm-i.d. Both columns are packed with the same stationary phase, spherical particles with 1-um diameter. A) When the columns were prepared, the figure shows that the column with the larger diameter has more packing irregularities. Explain this observation. B) Predict what affect this should have on band broadening and discuss your prediction using the van Deemter terms. C) Does this figure support your explanations in application question 33? Explain why or why not and make any changes in your answers in light of this figure. Figure 4 SEM images of sections of packed columns for a) 750 and b) 30-um-i.d. capillary columns.³

