unknown? 11. Triclosan is an antibacterial compound with an absorbance maximum at 293 nm. A student prepared a calibration curve by measuring five standard solutions of the triclosan in a cuvette with a path length of 5.00 cm. The calibration curve is shown below. (a) Determine the molar absorptivity of triclosan at 293 nm. (b) The absorbance of an unknown sample is measured at 293 nm in a 1.00 cm cell. The absorbance was 0.111. What is the concentration of triclosan in the unknown? (c) What is the absorbance and transmittance of a triclosan sample with a concentration of 0.000840 M measured in a 5.00 cm cuvette? Triclosan calibration curve Absorbance at 293 nm 0.25- 0.2- 0.15- 0.1- 0.05- 0 0 0.0002 0.0004 0.0006 0.0008 0.001 [triclosan] 0.0012 The equation for the line is y = 204.5x

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 13.24QAP
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unknown?
11. Triclosan is an antibacterial compound with an absorbance maximum at 293 nm.
A student prepared a calibration curve by measuring five standard solutions of the
triclosan in a cuvette with a path length of 5.00 cm. The calibration curve is
shown below. (a) Determine the molar absorptivity of triclosan at 293 nm.
(b) The absorbance of an unknown sample is measured at 293 nm in a 1.00 cm
cell. The absorbance was 0.111. What is the concentration of triclosan in the
unknown? (c) What is the absorbance and transmittance of a triclosan sample with
a concentration of 0.000840 M measured in a 5.00 cm cuvette?
Triclosan calibration curve
Absorbance at 293 nm
0.25-
0.2-
0.15-
0.1-
0.05-
0
0
0.0002 0.0004 0.0006 0.0008 0.001
[triclosan]
0.0012
The equation for the line
is y = 204.5x
Transcribed Image Text:unknown? 11. Triclosan is an antibacterial compound with an absorbance maximum at 293 nm. A student prepared a calibration curve by measuring five standard solutions of the triclosan in a cuvette with a path length of 5.00 cm. The calibration curve is shown below. (a) Determine the molar absorptivity of triclosan at 293 nm. (b) The absorbance of an unknown sample is measured at 293 nm in a 1.00 cm cell. The absorbance was 0.111. What is the concentration of triclosan in the unknown? (c) What is the absorbance and transmittance of a triclosan sample with a concentration of 0.000840 M measured in a 5.00 cm cuvette? Triclosan calibration curve Absorbance at 293 nm 0.25- 0.2- 0.15- 0.1- 0.05- 0 0 0.0002 0.0004 0.0006 0.0008 0.001 [triclosan] 0.0012 The equation for the line is y = 204.5x
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