What is the Rf value of each amino acid observed?What are the possible components of the amino acid mixture? Answer can be up to two amino acids per component. 3.) A mixture of amino acids was subjected to paper chromatography with six amino acid standardsin order to determine its composition. After visualization using ninhydrin, the measureddistances were as follows:Distances:GFSolvent - 10.3cmA - 4.5cmB - 1.0cmAC - 6.6cmDED - 3.9cmE - 3.5cmBF - 9.1cmG - 10.3cmX1 - 0.7cmX2 - 4.3cmX3 - 7.1cma.) What is the Rf value of each amino acid observed?b.) What are the possible components of the amino acid mixture? Answer can be up to two aminoacids per component.

Paper chromatography is a analytical method to separate the mixture of molecules with similar…

Answered: What is the Rf value of each amino acid…

What is the Rf value of each amino acid observed? What are the possible components of the amino acid mixture? Answer can be up to two amino acids per component.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Explain the Rf value obtained on the basis of the structure of amino acids use. Explain in 1-3 sentence only.
Consider an amino acid (A) with no ionizable side chains, and call the three species involved in the acid/base equilibria H2A+, HA, and A- (see scheme below). Assume that pKa(1) = 2.0 and that pKa(2) = 9.0. Suppose that the total concentration of the amino acid is 1.0 mM. Report to two significant digits. pH [H2A+] (mM) [HA] (mM) [A-] (mM) 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0