Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes. Estimate the osmotic pressure drop across the membrane of an animal cell in a 0.10 M NaCl solution, assuming that the internal total solute concentration is 0.36 osM and temperature is 30.0°C. Do you think that this pressure drop would lyse cells? Why or why not?

Cell lysis is a method in which the outer boundary is broken or disrupted in order to release inter…

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes.

Estimate the osmotic pressure drop across the membrane of an animal cell in a 0.10 M NaCl solution, assuming that the internal total solute concentration is 0.36 osM and temperature is 30.0°C. Do you think that this pressure drop would lyse cells? Why or why not?

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