The data below were collected during a passive membrane transport experiment where two solutes (A and B) were evaluated relative to their separate transport proteins. Transport rate (mmol/min) Solute A Solute B [S] mM (S) mM Transport rate (mmol/min) 2.0 0.3 2.0 0.5 4.0 0.7 4.0 1.0 6.0 1.0 6.0 1.4 8.0 1.3 8.0 1.6 10.0 1.5 10.0 1.7 12.0 1.7 12.0 1.8 14.0 1.9 14.0 1.9 16.0 2.0 16.0 2.0 18.0 2.0 18.0 2.0 4. Identify the Km for Solute B. Your answer must include the number with one decimal (similar to the numbers in the table) followed by a space and then the units 5. Which transport protein is present in the highest concentration? 6. Which ligand has the greatest affinity for its protein?
The data below were collected during a passive membrane transport experiment where two solutes (A and B) were evaluated relative to their separate transport proteins. Transport rate (mmol/min) Solute A Solute B [S] mM (S) mM Transport rate (mmol/min) 2.0 0.3 2.0 0.5 4.0 0.7 4.0 1.0 6.0 1.0 6.0 1.4 8.0 1.3 8.0 1.6 10.0 1.5 10.0 1.7 12.0 1.7 12.0 1.8 14.0 1.9 14.0 1.9 16.0 2.0 16.0 2.0 18.0 2.0 18.0 2.0 4. Identify the Km for Solute B. Your answer must include the number with one decimal (similar to the numbers in the table) followed by a space and then the units 5. Which transport protein is present in the highest concentration? 6. Which ligand has the greatest affinity for its protein?
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Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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![### Passive Membrane Transport Experiment
The data below were collected during a passive membrane transport experiment where two solutes (A and B) were evaluated relative to their separate transport proteins.
| Solute A [S] mM | Transport rate (mmol/min) | Solute B [S] mM | Transport rate (mmol/min) |
|------------------|----------------------------|-----------------|---------------------------|
| 2.0 | 0.3 | 2.0 | 0.5 |
| 4.0 | 0.7 | 4.0 | 1.0 |
| 6.0 | 1.0 | 6.0 | 1.4 |
| 8.0 | 1.3 | 8.0 | 1.6 |
|10.0 | 1.5 |10.0 | 1.7 |
|12.0 | 1.7 |12.0 | 1.8 |
|14.0 | 1.9 |14.0 | 1.9 |
|16.0 | 2.0 |16.0 | 2.0 |
|18.0 | 2.0 |18.0 | 2.0 |
**Questions:**
4. **Identify the Km for Solute B.** Your answer must include the number with one decimal (similar to the numbers in the table) followed by a space and then the units.
___________________________
5. **Which transport protein is present in the highest concentration?** ___________________________
6. **Which ligand has the greatest affinity for its protein?** ___________________________
---
**Explanation of Data Table:**
The table provides data for two solutes, A and B, including their concentrations in millimoles (mM) and corresponding transport rates in millimoles per minute (mmol/min). The transport rate of each solute increases with its concentration, but eventually reaches a maximum rate.
**Analysis:**
- The table can be used to determine the kinetic parameters such as the Km (Michaelis constant) and Vmax (maximum rate) for each solute.
- The transport rate can be plotted against solute concentration to visually determine these parameters.
Note:](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa5b6fab4-dfde-4ec5-a3de-a1d9022f0250%2F4bf62b36-fca7-4533-9ad9-a3a30e1ae9eb%2Fl4l669s_processed.png&w=3840&q=75)
Transcribed Image Text:### Passive Membrane Transport Experiment
The data below were collected during a passive membrane transport experiment where two solutes (A and B) were evaluated relative to their separate transport proteins.
| Solute A [S] mM | Transport rate (mmol/min) | Solute B [S] mM | Transport rate (mmol/min) |
|------------------|----------------------------|-----------------|---------------------------|
| 2.0 | 0.3 | 2.0 | 0.5 |
| 4.0 | 0.7 | 4.0 | 1.0 |
| 6.0 | 1.0 | 6.0 | 1.4 |
| 8.0 | 1.3 | 8.0 | 1.6 |
|10.0 | 1.5 |10.0 | 1.7 |
|12.0 | 1.7 |12.0 | 1.8 |
|14.0 | 1.9 |14.0 | 1.9 |
|16.0 | 2.0 |16.0 | 2.0 |
|18.0 | 2.0 |18.0 | 2.0 |
**Questions:**
4. **Identify the Km for Solute B.** Your answer must include the number with one decimal (similar to the numbers in the table) followed by a space and then the units.
___________________________
5. **Which transport protein is present in the highest concentration?** ___________________________
6. **Which ligand has the greatest affinity for its protein?** ___________________________
---
**Explanation of Data Table:**
The table provides data for two solutes, A and B, including their concentrations in millimoles (mM) and corresponding transport rates in millimoles per minute (mmol/min). The transport rate of each solute increases with its concentration, but eventually reaches a maximum rate.
**Analysis:**
- The table can be used to determine the kinetic parameters such as the Km (Michaelis constant) and Vmax (maximum rate) for each solute.
- The transport rate can be plotted against solute concentration to visually determine these parameters.
Note:
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