Example Problem: FRAP Data Interpretation The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity intens ROI A Time Time Post-bleaching imaging с Time D Time Pre-bleaching Bleaching imaging imaging (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
Example Problem: FRAP Data Interpretation The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP experiment with purified liposomes. The FRAP recovery curves are shown below. Fluorescence intensity intens ROI A Time Time Post-bleaching imaging с Time D Time Pre-bleaching Bleaching imaging imaging (a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D? (b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Example Problem: FRAP Data Interpretation
The diffusion rate of four different membrane proteins (A, B, C, and D) was measured using a FRAP
experiment with purified liposomes. The FRAP recovery curves are shown below.
Fluorescence
intensity
ROI
A
3
Time
Pre-bleaching Bleaching
imaging
imaging
B
Time
Post-bleaching
imaging
C
Time
D
Time
(a) Which membrane protein exhibits the higher rate of diffusion in the lipid bilayer? A or B? C or D?
(b) Explain the most likely cause of the difference in the recovery curves for proteins A and C.
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