Assume your eyes have a resolving power of 0.1 mm, which represents what you can see with a magnification of 1x. If an electron microscope is operating at a resolving power of 2 Å, what is its image magnification? Show all your work.
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- Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4,500 um Scanning (40X): 1.8 mm x 100X/40X = 5 mm = %3D Low power (100X): FOV diameter = 1.8 mm = 1,800 um 1.8 mm x 100X/400X = 0.45 mm =450 um 180 um High power (400X): 1.8 mm x 100X/1000x = 28 mm = Oil immersion (1000X): %3D Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification FOV diam. um FOV diam. um length um length umCalculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length umUsing a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
- The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400x
- Imagine you are using a microscope with 5x eye pieces (instead of 10x like ours). Complete the sentence. If you change from the 10x objective lens to the 40x objective lens, the total magnification changes from [Select] ř to | Select ] Select] 15x 50x 10x 梦 $ 99+For a microscope that has an objective lens magnification of 100X and an ocular lens magnification of 5X, calculate the total magnification. 500X O 1000X O 50X O 100X O No listed answer is correct.Imagine you are using a microscope with 20x eye pieces (instead of 10x like ours). Complete the sentence. If you change from the 10x objective lens to the 40x objective lens, the total magnification changes from [ Select ] to [ Select ]
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.AsapYou have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): Tick the right answers:o Objectives B and C are equivalent.o Objective D will allow more detailed observation than objective C.o To see small structures, you should choose objective D rather than C.o Objective D has a lower resolution than objective C.o To observe large objects, it is better to use objective B rather than objective C