Part B: Preparation of Standard Graph & Determination of Concentration of Original Unknown Indigo Carmine Solution (Don't Forget to Include Units!) 1. Equation for the Best-Fit Straight Line (from Standard Graph) Y=0.0002x+3E-19 2. Average Absorbance for Diluted Unknowns A3, B3 & 0.492nm C3 Calculation for Line 3 Below Using the Equation for the Best-Fit Straight Line (Show All Work!) 3. Concentration of KMNO4 in Diluted Unknowns A3, B3 & C3 5. Average of Actual Overall Dilution Factor for Diluted Unknowns A3, B3 & C3 6. Concentration of Original Unknown Sample of KMN04 First Dilution [1:10] A1 B1 C1 1. Actual Volume of Unknown KMN04 Solution Pipetted Into Tube 1.10 mL 1.05 ml 1.02 ml 2. Actual Volume of Distilled Water Pipetted Into Tube 8.91 mL 8.95 mL 8.97 mL 3. Total Volume of Solution In Tube 10.01mL 10ml 9.999mL Plot Area 4. Actual Dilution Factor 1.123 1.117 1.1147 Second Dilution [1:4] A2 B2 C2 5. Actual Volume of Diluted [1:10] KMnO4Solution Pipetted Into Tube 1.51 mL 1.49 mL 1.52 mL 6. Actual Volume of Distilled Water Pipetted Into Tube 4.48 ml 4.50 mL 4.48 mL 7. Total Volume of Solution In Tube 5.99ml 5.99mL 5.99mL 8. Actual Dilution Factor 3.95 3.95 3.95 Third Dilution [1:7] АЗ B3 C3 9. Actual Volume of Diluted [1:4] KMnO4Solution Pipetted Into Tube 1.51mL 1.49mL 1.52mL 10. Actual Volume of Distilled Water Pipetted Into Tube 4.49mL 4.51mL 4.48mL 11. Total Volume of Solution In Tube 6.00mL 6.00mL 6.00mL 12. Actual Dilution Factor 7.00 7.00 7.00 Overall Dilution [1:280] АЗ B3 C3 13. Actual Overall Dilution Factor 31.05 30.88 30.82 АЗ B3 C3 0.51 0.49 0.48
Part B: Preparation of Standard Graph & Determination of Concentration of Original Unknown Indigo Carmine Solution (Don't Forget to Include Units!) 1. Equation for the Best-Fit Straight Line (from Standard Graph) Y=0.0002x+3E-19 2. Average Absorbance for Diluted Unknowns A3, B3 & 0.492nm C3 Calculation for Line 3 Below Using the Equation for the Best-Fit Straight Line (Show All Work!) 3. Concentration of KMNO4 in Diluted Unknowns A3, B3 & C3 5. Average of Actual Overall Dilution Factor for Diluted Unknowns A3, B3 & C3 6. Concentration of Original Unknown Sample of KMN04 First Dilution [1:10] A1 B1 C1 1. Actual Volume of Unknown KMN04 Solution Pipetted Into Tube 1.10 mL 1.05 ml 1.02 ml 2. Actual Volume of Distilled Water Pipetted Into Tube 8.91 mL 8.95 mL 8.97 mL 3. Total Volume of Solution In Tube 10.01mL 10ml 9.999mL Plot Area 4. Actual Dilution Factor 1.123 1.117 1.1147 Second Dilution [1:4] A2 B2 C2 5. Actual Volume of Diluted [1:10] KMnO4Solution Pipetted Into Tube 1.51 mL 1.49 mL 1.52 mL 6. Actual Volume of Distilled Water Pipetted Into Tube 4.48 ml 4.50 mL 4.48 mL 7. Total Volume of Solution In Tube 5.99ml 5.99mL 5.99mL 8. Actual Dilution Factor 3.95 3.95 3.95 Third Dilution [1:7] АЗ B3 C3 9. Actual Volume of Diluted [1:4] KMnO4Solution Pipetted Into Tube 1.51mL 1.49mL 1.52mL 10. Actual Volume of Distilled Water Pipetted Into Tube 4.49mL 4.51mL 4.48mL 11. Total Volume of Solution In Tube 6.00mL 6.00mL 6.00mL 12. Actual Dilution Factor 7.00 7.00 7.00 Overall Dilution [1:280] АЗ B3 C3 13. Actual Overall Dilution Factor 31.05 30.88 30.82 АЗ B3 C3 0.51 0.49 0.48
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Only need help on part b!! Please
![Part B: Preparation of Standard Graph & Determination of Concentration of
Original Unknown Indigo Carmine Solution (Don't Forget to Include Units!)
1. Equation for the Best-Fit Straight Line (from Standard
Graph)
Y=0.0002x+3E-19
2. Average Absorbance for Diluted Unknowns A3, B3 &
0.492nm
C3
Calculation for Line 3 Below Using the Equation for the Best-Fit Straight Line (Show All Work!)
3. Concentration of KMNO4 in Diluted Unknowns A3, B3
& C3
5. Average of Actual Overall Dilution Factor for Diluted
Unknowns A3, B3 & C3
6. Concentration of Original Unknown Sample of KMN04
First Dilution [1:10]
A1
B1
C1
1. Actual Volume of Unknown KMN04 Solution Pipetted Into Tube
1.10 mL
1.05 ml
1.02 ml
2. Actual Volume of Distilled Water Pipetted Into Tube
8.91 mL
8.95 mL
8.97 mL
3. Total Volume of Solution In Tube
10.01mL
10ml
9.999mL
Plot Area
4. Actual Dilution Factor
1.123
1.117
1.1147
Second Dilution [1:4]
A2
B2
C2
5. Actual Volume of Diluted [1:10] KMnO4Solution Pipetted Into
Tube
1.51 mL
1.49 mL
1.52 mL
6. Actual Volume of Distilled Water Pipetted Into Tube
4.48 ml
4.50 mL
4.48 mL
7. Total Volume of Solution In Tube
5.99ml
5.99mL
5.99mL
8. Actual Dilution Factor
3.95
3.95
3.95
Third Dilution [1:7]
АЗ
B3
C3
9. Actual Volume of Diluted [1:4] KMnO4Solution Pipetted Into
Tube
1.51mL
1.49mL
1.52mL
10. Actual Volume of Distilled Water Pipetted Into Tube
4.49mL
4.51mL
4.48mL
11. Total Volume of Solution In Tube
6.00mL
6.00mL
6.00mL
12. Actual Dilution Factor
7.00
7.00
7.00
Overall Dilution [1:280]
АЗ
B3
C3
13. Actual Overall Dilution Factor
31.05
30.88
30.82
АЗ
B3
C3
0.51
0.49
0.48](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbd285f6f-67ec-4ffa-8e9b-700f12fa780d%2Fd89fd2af-4f69-4127-9633-bdc9a8fc80d0%2F9913csc.jpeg&w=3840&q=75)
Transcribed Image Text:Part B: Preparation of Standard Graph & Determination of Concentration of
Original Unknown Indigo Carmine Solution (Don't Forget to Include Units!)
1. Equation for the Best-Fit Straight Line (from Standard
Graph)
Y=0.0002x+3E-19
2. Average Absorbance for Diluted Unknowns A3, B3 &
0.492nm
C3
Calculation for Line 3 Below Using the Equation for the Best-Fit Straight Line (Show All Work!)
3. Concentration of KMNO4 in Diluted Unknowns A3, B3
& C3
5. Average of Actual Overall Dilution Factor for Diluted
Unknowns A3, B3 & C3
6. Concentration of Original Unknown Sample of KMN04
First Dilution [1:10]
A1
B1
C1
1. Actual Volume of Unknown KMN04 Solution Pipetted Into Tube
1.10 mL
1.05 ml
1.02 ml
2. Actual Volume of Distilled Water Pipetted Into Tube
8.91 mL
8.95 mL
8.97 mL
3. Total Volume of Solution In Tube
10.01mL
10ml
9.999mL
Plot Area
4. Actual Dilution Factor
1.123
1.117
1.1147
Second Dilution [1:4]
A2
B2
C2
5. Actual Volume of Diluted [1:10] KMnO4Solution Pipetted Into
Tube
1.51 mL
1.49 mL
1.52 mL
6. Actual Volume of Distilled Water Pipetted Into Tube
4.48 ml
4.50 mL
4.48 mL
7. Total Volume of Solution In Tube
5.99ml
5.99mL
5.99mL
8. Actual Dilution Factor
3.95
3.95
3.95
Third Dilution [1:7]
АЗ
B3
C3
9. Actual Volume of Diluted [1:4] KMnO4Solution Pipetted Into
Tube
1.51mL
1.49mL
1.52mL
10. Actual Volume of Distilled Water Pipetted Into Tube
4.49mL
4.51mL
4.48mL
11. Total Volume of Solution In Tube
6.00mL
6.00mL
6.00mL
12. Actual Dilution Factor
7.00
7.00
7.00
Overall Dilution [1:280]
АЗ
B3
C3
13. Actual Overall Dilution Factor
31.05
30.88
30.82
АЗ
B3
C3
0.51
0.49
0.48
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