A researcher is isolating proteins from a cell via different techniques. They find that when the cell is ground with a blender, all of the proteins in the cell can be extracted. However if instead the cell membranes are agitated in a 1.2 M KCl mixture, only about 70% of the total protein is extracted. What class of membrane proteins are in this soluble extract and what forces normally hold them to the membrane? What class of membrane proteins are the 30% of proteins that remain behind, and what forces hold these proteins in the membrane? If a solution of ionic solids was not available, what other solvent systems could be used to extract these proteins?

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Q: Consider two proteins, A and B, that can interact with a Kg = 2.55 nM. If you mix the two proteins…

A: Option A is the answer. Given,Kd = 2.55nM Kd = [A][B]/[AB] = 2.55

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A:

Q: In isoelectric focusing, a protein sample is applied at the end of a gel with an immobilized pH…

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Q: SDS-PAGE gels separate proteins by charge.  Therefore proteins that are 10,000 daltons (g/mol) will…

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Q: Using the chart below, can you produce a two-step procedure that demonstrates protein purification…

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Q: Given that the protein concentration is 0.25 μM, what is the association constant (KA) of the…

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Q: Both milk and egg whites (albumin) contain proteins. What do your findings suggest for the stability…

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.