Choose one: O A. The hyaluronan would show a single band at ~8 x 106 daltons, but the ribosome would show a smear with a wide range of molecular weights. O B. Both substances would show smears with wide ranges of molecular weights. O C. The ribosome would show a single band at ~2.5 x 106 daltons, but the hyaluronan would show a smear with a wide range of molecular weights. O D. Each would show a single band reflecting the molecular weight: ~2.5 x 106 daltons for the ribosome and ~8 x 106 daltons for hyaluronan.
Choose one: O A. The hyaluronan would show a single band at ~8 x 106 daltons, but the ribosome would show a smear with a wide range of molecular weights. O B. Both substances would show smears with wide ranges of molecular weights. O C. The ribosome would show a single band at ~2.5 x 106 daltons, but the hyaluronan would show a smear with a wide range of molecular weights. O D. Each would show a single band reflecting the molecular weight: ~2.5 x 106 daltons for the ribosome and ~8 x 106 daltons for hyaluronan.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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Transcribed Image Text:Choose one:
O A. The hyaluronan would show a single band at ~8 x 106 daltons, but the ribosome would show a smear with a
wide range of molecular weights.
B. Both substances would show smears with wide ranges of molecular weights.
C. The ribosome would show a single band at ~2.5 x 106 daltons, but the hyaluronan would show a smear with a
wide range of molecular weights.
D. Each would show a single band reflecting the molecular weight: ~2.5 x 106 daltons for the ribosome and ~8 x
106 daltons for hyaluronan.
Transcribed Image Text:As described in the figure below, the technique of ultracentrifugation can be used to demonstrate whether a substance is composed of
large covalently linked molecules, or smaller molecules held together in a complex by noncovalent bonds.
You are studying two substances that both have very high molecular weights: hyaluronan, a carbohydrate found in the extracellular
matrix with a MW of ~8 x 106 daltons, and the bacterial ribosome, with a MW of -2.5 x 106. Hyaluronan is a single, long-chain polymer,
while the ribosome subunit is a complex of 55 different proteins plus three long RNA molecules. Which of the following describes the
pattern you would expect to see from ultracentrifugation of these two substances?
the sample loaded as a
narrow band at the top of
the tube
(A)
sample
CENTRIFUGATION
stabilizing
sucrose
gradient
tube
heterogeneous
aggregates would
sediment to
produce a
diffuse smear
hemoglobin
protein
sediments as a
single band
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