Overview In this activity, you will have a chance to simulate diffusion and osmosis using dialysis tubing. Dialysis tubing has small pores throughout that will only allow particles of a certain size to pass through, effectively blocking particles that are too large. In this experiment, dialysis tubing will be used to simulate a cell membrane. Cell Homeostasis Simulation Lab Instructions 1. On a separate piece of paper draw the following 6x6 table. You will be submitting your data to this assignment and using this information to answer some questions in the associated lab report. Beaker A B C D E % sugar solution (beakers) % sugar solution (dialysis tubes) Initial mass (g) Final mass (g) Difference between masses (final mass - initial mass) 2. When entering your data: 。 Round your answers to the nearest 10th. (Eg. 16.5879 should be recorded as 16.6) • Be careful when calculating the difference between the final mass and the initial mass. Make sure you subtract the final mass from the initial mass and if the result is negative place the negative sign in your answer. (ex. initial mass=5 and the final mass=3, so the difference is -2, so you should enter -2.0 as your answer. 3. Open the Cell Homeostasis lab and click on the "Start" button, and click "Continue". 4. You will start by creating a control beaker labeled "A" by adding 1,000 mL of water to the beaker. 5. Follow the prompts and add 1,000 mL of water to each beaker. 6. After beaker "C" you will add different masses (g) of sugar to each beaker. To add sugar: ■ Place the petri dish onto the scale and tare (zero) the mass of the petri dish. ■ Add the grams of sugar indicated for the solution. initial mass) 2. When entering your data: 。 Round your answers to the nearest 10th. (Eg. 16.5879 should be recorded as 16.6) • Be careful when calculating the difference between the final mass and the initial mass. Make sure you subtract the final mass from the initial mass and if the result is negative place the negative sign in your answer. (ex. initial mass=5 and the final mass=3, so the difference is -2, so you should enter -2.0 as your answer. 3. Open the Cell Homeostasis lab and click on the "Start" button, and click "Continue". 4. You will start by creating a control beaker labeled "A" by adding 1,000 mL of water to the beaker. 5. Follow the prompts and add 1,000 mL of water to each beaker. 6. After beaker "C" you will add different masses (g) of sugar to each beaker. To add sugar: ■ Place the petri dish onto the scale and tare (zero) the mass of the petri dish. ■ Add the grams of sugar indicated for the solution. ■ Place the sugar into the beaker and stir. ■ Repeat for beakers "D" and "E". Don't forget to tare each time. 7. Record the % sugar solution for each beaker in your table. 8. Prepare to add prefilled dialysis tubes (represent cells) to each beaker. ■ The control dialysis tube has been filled with water only. ■ Tubes B-E is filled with different % sugar solutions. Record the information in your table. 9. Weigh each dialysis tube (A-E) and place it in its proper beaker. Make sure to record the initial mass (data) of your tubes on your table. 10. Dialysis tubes will remain in the beakers for "24hrs" 11. After the 24 hours have passed, record the final mass (data) of your dialysis tubes (A-E) and place the information on your table. 12. Calculate the difference between your initial and final masses by subtracting the initial from the final mass for each dialysis tube (A-E) and record the information on your table in the final row. Make sure to include positive and negative numbers.