Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities will result in the expression of the cloned gene with the highest efficiency? Stul: 5'-AGG|CCT-3 3'-TCC GGA-5 SalI cuts: 5 -G|TCGAC-3' 3'-CAGCT|G-5' ECORI: 5'-G|AATTC-3' 3'-CTTAA|G-5' Promoter P StyL_ Sall EcoRI Eco RI Stul Start codon Sll Vector 2 TCCGGCGGAATTCCAAGGCCT SAGGTTCCGGE psy2 CGTCGACTCCGGC 3' GCAGCTGAGGCCG 5'

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities
will result in the expression of the cloned gene with the highest efficiency?
Stul: 5'-AGG | CCT-3'
3'-TCC|GGA-5
SalI cuts: 5'-G|TCGAC-3'
3'-CAGCT|G-5'
ECORI: 5'-G| AATTC-3'
3'-CTTAA|G-5'
Promoter P Styl Sall
EcoRI
Eco RI
Stul
Start codon
Sall
Vector 2
5' TCCGGCGGAATTCCAAGGCCT
3 AGGCCGCCTTAAGGTTCCGGA
psy2
CGTCGACTCCGGC
GCAGCTGAGGCG 5'
Transcribed Image Text:Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities will result in the expression of the cloned gene with the highest efficiency? Stul: 5'-AGG | CCT-3' 3'-TCC|GGA-5 SalI cuts: 5'-G|TCGAC-3' 3'-CAGCT|G-5' ECORI: 5'-G| AATTC-3' 3'-CTTAA|G-5' Promoter P Styl Sall EcoRI Eco RI Stul Start codon Sall Vector 2 5' TCCGGCGGAATTCCAAGGCCT 3 AGGCCGCCTTAAGGTTCCGGA psy2 CGTCGACTCCGGC GCAGCTGAGGCG 5'
O A. Digestion of both component with Stul and Sall
O B. Digestion of vector with Sall and PCR product with Stul
O C. Digestion of both components with EcoRI and Sall
O D. Digestion of vector and PCR product with Stul
E. Digestion of both components with EcoRI and Stul
Transcribed Image Text:O A. Digestion of both component with Stul and Sall O B. Digestion of vector with Sall and PCR product with Stul O C. Digestion of both components with EcoRI and Sall O D. Digestion of vector and PCR product with Stul E. Digestion of both components with EcoRI and Stul
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