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Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities will result in the expression of the cloned gene with the highest efficiency? Stul: 5'-AGG|CCT-3 3'-TCC GGA-5 SalI cuts: 5 -G|TCGAC-3' 3'-CAGCT|G-5' ECORI: 5'-G|AATTC-3' 3'-CTTAA|G-5' Promoter P StyL_ Sall EcoRI Eco RI Stul Start codon Sll Vector 2 TCCGGCGGAATTCCAAGGCCT SAGGTTCCGGE psy2 CGTCGACTCCGGC 3' GCAGCTGAGGCCG 5'

Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities will result in the expression of the cloned gene with the highest efficiency? Stul: 5'-AGG|CCT-3 3'-TCC GGA-5 SalI cuts: 5 -G|TCGAC-3' 3'-CAGCT|G-5' ECORI: 5'-G|AATTC-3' 3'-CTTAA|G-5' Promoter P StyL_ Sall EcoRI Eco RI Stul Start codon Sll Vector 2 TCCGGCGGAATTCCAAGGCCT SAGGTTCCGGE psy2 CGTCGACTCCGGC 3' GCAGCTGAGGCCG 5'

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities will result in the expression of the cloned gene with the highest efficiency? Stul: 5'-AGG | CCT-3' 3'-TCC|GGA-5 SalI cuts: 5'-G|TCGAC-3' 3'-CAGCT|G-5' ECORI: 5'-G| AATTC-3' 3'-CTTAA|G-5' Promoter P Styl Sall EcoRI Eco RI Stul Start codon Sall Vector 2 5' TCCGGCGGAATTCCAAGGCCT 3 AGGCCGCCTTAAGGTTCCGGA psy2 CGTCGACTCCGGC GCAGCTGAGGCG 5'
Transcribed Image Text:Using the following expression vector and the insert of interest with flaking region (PCR product of gene Psy2 and flanking region), which of these possibilities will result in the expression of the cloned gene with the highest efficiency? Stul: 5'-AGG | CCT-3' 3'-TCC|GGA-5 SalI cuts: 5'-G|TCGAC-3' 3'-CAGCT|G-5' ECORI: 5'-G| AATTC-3' 3'-CTTAA|G-5' Promoter P Styl Sall EcoRI Eco RI Stul Start codon Sall Vector 2 5' TCCGGCGGAATTCCAAGGCCT 3 AGGCCGCCTTAAGGTTCCGGA psy2 CGTCGACTCCGGC GCAGCTGAGGCG 5'
O A. Digestion of both component with Stul and Sall O B. Digestion of vector with Sall and PCR product with Stul O C. Digestion of both components with EcoRI and Sall O D. Digestion of vector and PCR product with Stul E. Digestion of both components with EcoRI and Stul
Transcribed Image Text:O A. Digestion of both component with Stul and Sall O B. Digestion of vector with Sall and PCR product with Stul O C. Digestion of both components with EcoRI and Sall O D. Digestion of vector and PCR product with Stul E. Digestion of both components with EcoRI and Stul
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