Phase 1 Isolated Colonies Present? Yes, many Countable? Too numerous to count Too numerous to count Countable Too few to count Plate # Dilution Label Growth Appearance on Plate 1 1:10,000 Heavy No 2 1:100,000 Heavy Yes, few 3 1:1,000,000 Intermediate 1:10,000,000 Scant Image Yes, few Save image Phase 2 Plate Selected For Counting Volume of Diluted Sample (mL) Sample Dilution Colony Count How to Calculate Bacterial Concentration in Original Sample Plate 3 1 1:1,000,000 52 Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample. Number of CFUs = CFUs You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original undiluted sample. Number of CFUs = CFUs
Phase 1 Isolated Colonies Present? Yes, many Countable? Too numerous to count Too numerous to count Countable Too few to count Plate # Dilution Label Growth Appearance on Plate 1 1:10,000 Heavy No 2 1:100,000 Heavy Yes, few 3 1:1,000,000 Intermediate 1:10,000,000 Scant Image Yes, few Save image Phase 2 Plate Selected For Counting Volume of Diluted Sample (mL) Sample Dilution Colony Count How to Calculate Bacterial Concentration in Original Sample Plate 3 1 1:1,000,000 52 Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample. Number of CFUs = CFUs You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original undiluted sample. Number of CFUs = CFUs
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.4: The Gram Stain
Problem 5RQ
Related questions
Question
Transcribed Image Text:Phase 1
Isolated Colonies Present?
Yes, many
Countable?
Too numerous to count
Too numerous to count
Countable
Too few to count
Plate #
Dilution Label
Growth Appearance on Plate
1
1:10,000
Heavy
No
2
1:100,000
Heavy
Yes, few
3
1:1,000,000
Intermediate
1:10,000,000
Scant
Image
Yes, few
Save image
Phase 2
Plate Selected For Counting
Volume of Diluted Sample (mL)
Sample Dilution
Colony Count
How to Calculate Bacterial Concentration in Original Sample
Plate 3
1
1:1,000,000
52
Transcribed Image Text:Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample.
Number of CFUs =
CFUs
You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and
observational data you acquired, re-calculate the number of CFUs in the original undiluted sample.
Number of CFUs =
CFUs
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