Two of the bypass reactions of gluconeogenesis involve: phosphorylation of the metabolite using phosphate from Pi. ☐ phosphorylation of the metabolite using phosphate from ATP. dephosphorylation of the metabolite without using it to make ATP. phosphorylation of ADP using phosphate from the metabolite.
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- Two of the bypass reactions of gluconeogenesis involve: a phosphorylation of ADP using phosphate from the metabolite. b phosphorylation of the metabolite using phosphate from ATP. c dephosphorylation of the metabolite without using it to make ATP. d phosphorylation of the metabolite using phosphate from Pi.In this transamination reaction (right), which of the following are the products X and Y? Oxaloacetate Glutamate A Alanine, a-ketoglutarate B Aspartate, a-ketoglutarate C Glutamate, alanine D Pyruvate, aspartateConsider the following reaction sequence. glucose 1-phosphate + UTP UDP-glucose +-0-P-0- UDP-glucose + (glucose), UDP + (glucose),+1 Which of the following characterizes this process? consumes energy O all of these anabolic O a step in gluconeogenesis
- Describe the gluconeogenesis. What are the substrates for gluconeogenesis? (yellow boxes) and write the names of the enzymes belong to the gluconeogenic pathway. Glucose 4 se-6-phosphate usphoglucose isomerase Fructose-6-phosphate Fructose-1,6-bisphosphate HAidolase Triose phosphate Dihydroxyacetone phosphate isomerase Glyceraldehyde 3-phosphate P.. NAD CH,OH Glyceraldehyde 3-phosphate dehydrogenase 1,3-Bisphosphoglycerate CH,OPO,- NADH ADP Phosphoglycerate kinase ATP 3-Phosphoglycerate Phosphoglycerate || mutase 2-Phosphoglycerate 2 X Enolase Phosphoenolpyruvate GDP, CO, GIP Oxaloacetate ADP + P, 1 ATP, HCOS PyruvateExplain the fate of electrons that are removed during the oxidation of triose phosphates during the glycolysis.Also explain the glucose be "primed" with a high energy phosphate bond before it can be degraded in the glycolytic pathway.Which of the following cellular concentrations would be likely to increase the rate of glycolysis? Low AMP levels High glucagon levels ✓Low fructose-2,6-bisphosphate levels High AMP levels High phosphoenolpyruvate levels Low ATP levels
- An individual with chronic hypoglycemia was suspected of having a defect in one of the enzymes unique to gluconeogenesis. To identify the defective enzyme, tissue samples from a normal liver were compared to samples from the patient's liver biopsy, using a biochemical assay that measures glucose production from glycerol or malate. It was found that incubation with glycerol produced normal amounts of glucose in both the control and biopsied liver samples; however, incubation with malate did not lead to glucose production in the liver biopsy, even though it did lead to glucose production in the control liver sample. Based on these observations which of the 4 unique gluconeogenesis enzymes is most likely defective in the individual? Consider each enzyme and explain your choice, including why you ruled out enzymes that you did not choose. To answer this question review how glycerol enters gluconeogenesis (figure 9.48) and how citrate cycle metabolites are transported in and out of the…Identify the 4 steps of gluconeogenesis that are different from glycolysis. Write the reactants, enzymes, cofactors/coenzymes and products involved in the gluconeogenesis pathway.For each of the following situations, predict the major concequences for glycogen metabolism. Explain your logic in a few sentences. Will the amount of glycogen be higher or lower than the normal or unchanged. a) Effect of mutation Y194F in glycogenenin on glycogen synthesis. b) Effect on the loss of the gene that encodes inhibitor I of protein phosphatase I on both glycogen phosphorylase and glycogen synthase. c) Effect on the substrate analog shown below in glycogen synthesis (hint: what is the normal reaction sequence? At which step in the pathway does this sequence become blocked and why?)
- In the following diagram of glycolysis. Identify the steps that are amphibolic, taking place in both glycolysis and gluconeogenesis. Also, identify the regulated steps and what compounds act as regulators.What glycolytic intermediate is fructose converted to in the muscle, such that it can be utilized in glycolysis? Glucose 6-phosphate Fructose 6-phosphate Glyceraldehyde 3-phosphate Dihydroxyacetone phosphateIn which of the following metabolic conversions is ATP “generated” during glycolysis? Glucose —> Glucose-6-phosphate 1,3-Bisphosphoglycerate —> 3-Phosphoglycerate Fructose-1,6-bisphosphate —> Dihydroxyacetone phosphate + Glyceraldehyde-3-phosphate Glucose-6-phosphate —> Fructose-6-phosphate 2-Phosphoglycerate —> 3-Phosphoglycerate