The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase. H2O + CO2--> HCO3 + H+ Carbon Dioxide Concentration (mmol L-') 1.25 2.5 5.0 20.0 1/Velocity (M'sec) 36 x 10³ 20 x 10³ 12 x 10³ 6 x 10³ [H. DeVoe and G. B. Kistiakowsky, J. Am. Chem. Soc. 1961 83 (2), 274-280.] Use Excel (or similar) to generate a Line Weaver-Burke plot, then use the equation for a linear-fitted trend line to calculate the substrate affinity of CO2 for carbonic anhydrase. Answer in units of mM. Enter numerical values only, no letters or symbols. Round answer to 2 significant figures.
The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase. H2O + CO2--> HCO3 + H+ Carbon Dioxide Concentration (mmol L-') 1.25 2.5 5.0 20.0 1/Velocity (M'sec) 36 x 10³ 20 x 10³ 12 x 10³ 6 x 10³ [H. DeVoe and G. B. Kistiakowsky, J. Am. Chem. Soc. 1961 83 (2), 274-280.] Use Excel (or similar) to generate a Line Weaver-Burke plot, then use the equation for a linear-fitted trend line to calculate the substrate affinity of CO2 for carbonic anhydrase. Answer in units of mM. Enter numerical values only, no letters or symbols. Round answer to 2 significant figures.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic
anhydrase.
H2O + CO2--> HCO3 + H+
Carbon Dioxide Concentration
(mmol L-')
1.25
2.5
5.0
20.0
1/Velocity
(M'sec)
36 x 10³
20 x 10³
12 x 10³
6 x 10³
[H. DeVoe and G. B. Kistiakowsky, J. Am. Chem. Soc. 1961 83 (2), 274-280.]
Use Excel (or similar) to generate a Line Weaver-Burke plot, then use the equation for a linear-fitted trend line to calculate the substrate affinity of CO2 for carbonic
anhydrase. Answer in units of mM.
Enter numerical values only, no letters or symbols. Round answer to 2 significant figures.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0929a000-131e-4378-97de-16d36c27aaaf%2Fbda0c88e-1331-46fa-80ae-7640ede79aff%2Fmka58al_processed.png&w=3840&q=75)
Transcribed Image Text:The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic
anhydrase.
H2O + CO2--> HCO3 + H+
Carbon Dioxide Concentration
(mmol L-')
1.25
2.5
5.0
20.0
1/Velocity
(M'sec)
36 x 10³
20 x 10³
12 x 10³
6 x 10³
[H. DeVoe and G. B. Kistiakowsky, J. Am. Chem. Soc. 1961 83 (2), 274-280.]
Use Excel (or similar) to generate a Line Weaver-Burke plot, then use the equation for a linear-fitted trend line to calculate the substrate affinity of CO2 for carbonic
anhydrase. Answer in units of mM.
Enter numerical values only, no letters or symbols. Round answer to 2 significant figures.
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