Homework Help is Here – Start Your Trial Now!
Science
Biology
Three experiments demonstrating gene silencing (a) Fire/Mello: injection of dsRNA (b) Jorgensen: insertion of transgene (c) Baulcombe: insertion of viral gene 1. unc-22 dsRNA synthesized in lab. 1. Transgene inserted into petunia cells. 1. Viral gene inserted into tobacco plant. viral gene unc-22 Gene Antisense Transgene Gene dsRNA Sense Endogenous pigment gene 2. dsRNA injected into C. elegans embryos. 2. Adults grown from transformed cells have white sectors in flowers. 2. Plant exposed to virus but remains healthy. Micropipette with dsRNA solution 3. Adults display muscle defects. Conclusion. unc-22 gene silenced. Conclusion: viral gene silenced. Conclusion: transgene and endogenous pigment gene silenced. FIGURE 8-20 Three experiments reveal key features of gene silencing. (a) Fire and Mello demonstrated that dsRNA copies can selectively silence genes in C. elegans. (b) Jorgensen discovered that a transgene can silence an endogenous petunia gene necessary for floral color. (c) Baulcombe showed that plants with a copy of a viral transgene wore resistant to viral infection and produced SIRNAS complementary to the viral genome. laboratory and was composed of a sense (coding) RNA strand and a complementary antisense RNA strand. In their initial experiment, Fire and Mello injected dsRNA copies of the unc-22 gene into C. elegans embryos and watched as the embryos grew into adults that twitched and had muscle defects. This result was exciting because uno-22 was known to encode a muscle protein and null mutants of unc-22 displayed the same twitching and muscle defects. Taken together, these observations indi- cated that the injected dsRNA prevented the production of the Unc-22 protein. For their discovery of a new way to silence genes, Fire and Mello were awarded the Nobel Prize in Medicine or Physiology in 2004.

Three experiments demonstrating gene silencing (a) Fire/Mello: injection of dsRNA (b) Jorgensen: insertion of transgene (c) Baulcombe: insertion of viral gene 1. unc-22 dsRNA synthesized in lab. 1. Transgene inserted into petunia cells. 1. Viral gene inserted into tobacco plant. viral gene unc-22 Gene Antisense Transgene Gene dsRNA Sense Endogenous pigment gene 2. dsRNA injected into C. elegans embryos. 2. Adults grown from transformed cells have white sectors in flowers. 2. Plant exposed to virus but remains healthy. Micropipette with dsRNA solution 3. Adults display muscle defects. Conclusion. unc-22 gene silenced. Conclusion: viral gene silenced. Conclusion: transgene and endogenous pigment gene silenced. FIGURE 8-20 Three experiments reveal key features of gene silencing. (a) Fire and Mello demonstrated that dsRNA copies can selectively silence genes in C. elegans. (b) Jorgensen discovered that a transgene can silence an endogenous petunia gene necessary for floral color. (c) Baulcombe showed that plants with a copy of a viral transgene wore resistant to viral infection and produced SIRNAS complementary to the viral genome. laboratory and was composed of a sense (coding) RNA strand and a complementary antisense RNA strand. In their initial experiment, Fire and Mello injected dsRNA copies of the unc-22 gene into C. elegans embryos and watched as the embryos grew into adults that twitched and had muscle defects. This result was exciting because uno-22 was known to encode a muscle protein and null mutants of unc-22 displayed the same twitching and muscle defects. Taken together, these observations indi- cated that the injected dsRNA prevented the production of the Unc-22 protein. For their discovery of a new way to silence genes, Fire and Mello were awarded the Nobel Prize in Medicine or Physiology in 2004.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Topic Video
Question

In Figure 8-20, three very different situations are shown
that all result in gene silencing. What do these situations
have in common to make this is possible?

Three experiments demonstrating gene silencing (a) Fire/Mello: injection of dsRNA (b) Jorgensen: insertion of transgene (c) Baulcombe: insertion of viral gene 1. unc-22 dsRNA synthesized in lab. 1. Transgene inserted into petunia cells. 1. Viral gene inserted into tobacco plant. viral gene unc-22 Gene Antisense Transgene Gene dsRNA Sense Endogenous pigment gene 2. dsRNA injected into C. elegans embryos. 2. Adults grown from transformed cells have white sectors in flowers. 2. Plant exposed to virus but remains healthy. Micropipette with dsRNA solution 3. Adults display muscle defects. Conclusion. unc-22 gene silenced. Conclusion: viral gene silenced. Conclusion: transgene and endogenous pigment gene silenced. FIGURE 8-20 Three experiments reveal key features of gene silencing. (a) Fire and Mello demonstrated that dsRNA copies can selectively silence genes in C. elegans. (b) Jorgensen discovered that a transgene can silence an endogenous petunia gene necessary for floral color. (c) Baulcombe showed that plants with a copy of a viral transgene wore resistant to viral infection and produced SIRNAS complementary to the viral genome. laboratory and was composed of a sense (coding) RNA strand and a complementary antisense RNA strand. In their initial experiment, Fire and Mello injected dsRNA copies of the unc-22 gene into C. elegans embryos and watched as the embryos grew into adults that twitched and had muscle defects. This result was exciting because uno-22 was known to encode a muscle protein and null mutants of unc-22 displayed the same twitching and muscle defects. Taken together, these observations indi- cated that the injected dsRNA prevented the production of the Unc-22 protein. For their discovery of a new way to silence genes, Fire and Mello were awarded the Nobel Prize in Medicine or Physiology in 2004.
Transcribed Image Text:Three experiments demonstrating gene silencing (a) Fire/Mello: injection of dsRNA (b) Jorgensen: insertion of transgene (c) Baulcombe: insertion of viral gene 1. unc-22 dsRNA synthesized in lab. 1. Transgene inserted into petunia cells. 1. Viral gene inserted into tobacco plant. viral gene unc-22 Gene Antisense Transgene Gene dsRNA Sense Endogenous pigment gene 2. dsRNA injected into C. elegans embryos. 2. Adults grown from transformed cells have white sectors in flowers. 2. Plant exposed to virus but remains healthy. Micropipette with dsRNA solution 3. Adults display muscle defects. Conclusion. unc-22 gene silenced. Conclusion: viral gene silenced. Conclusion: transgene and endogenous pigment gene silenced. FIGURE 8-20 Three experiments reveal key features of gene silencing. (a) Fire and Mello demonstrated that dsRNA copies can selectively silence genes in C. elegans. (b) Jorgensen discovered that a transgene can silence an endogenous petunia gene necessary for floral color. (c) Baulcombe showed that plants with a copy of a viral transgene wore resistant to viral infection and produced SIRNAS complementary to the viral genome. laboratory and was composed of a sense (coding) RNA strand and a complementary antisense RNA strand. In their initial experiment, Fire and Mello injected dsRNA copies of the unc-22 gene into C. elegans embryos and watched as the embryos grew into adults that twitched and had muscle defects. This result was exciting because uno-22 was known to encode a muscle protein and null mutants of unc-22 displayed the same twitching and muscle defects. Taken together, these observations indi- cated that the injected dsRNA prevented the production of the Unc-22 protein. For their discovery of a new way to silence genes, Fire and Mello were awarded the Nobel Prize in Medicine or Physiology in 2004.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Mitochondrial mutations
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS