Genetic Variation
Genetic variation refers to the variation in the genome sequences between individual organisms of a species. Individual differences or population differences can both be referred to as genetic variations. It is primarily caused by mutation, but other factors such as genetic drift and sexual reproduction also play a major role.
Quantitative Genetics
Quantitative genetics is the part of genetics that deals with the continuous trait, where the expression of various genes influences the phenotypes. Thus genes are expressed together to produce a trait with continuous variability. This is unlike the classical traits or qualitative traits, where each trait is controlled by the expression of a single or very few genes to produce a discontinuous variation.
How can you assess dominance and mapping with only one mutant?
 alt="Using a new model lizard with a wild-type strain and genome sequence, you identify a
single mutant (the first mutant strain in this organism) that is defective in scale generation.
Transgenesis and CRISPR-Cas9 genome editing are possible in this organism. What
order of steps (shown below) will be the best approach to identify the gene mutated in this
strain?
(1) Sequencing of wild-type and mutant strain genomes
(2) Mapping
(3) Complementation
(4) Transgenic rescue of the mutant phenotype by the wild-type gene
(5) Assess dominance
(6) CRISPR of the gene in the wild-type strain
A. (1), (2), (3), (4), (5), (6)
B. (2), (6) - Skip other steps
C. (5), (2), (1), (6), (4) - Skip step 3
D. (5), (3), (2), (1), (6), (4)
E. (1), (6), (4) - Skip other steps
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