How can you assess dominance and mapping with only one mutant?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question

How can you assess dominance and mapping with only one mutant?

Using a new model lizard with a wild-type strain and genome sequence, you identify a
single mutant (the first mutant strain in this organism) that is defective in scale generation.
Transgenesis and CRISPR-Cas9 genome editing are possible in this organism. What
order of steps (shown below) will be the best approach to identify the gene mutated in this
strain?
(1) Sequencing of wild-type and mutant strain genomes
(2) Mapping
(3) Complementation
(4) Transgenic rescue of the mutant phenotype by the wild-type gene
(5) Assess dominance
(6) CRISPR of the gene in the wild-type strain
A. (1), (2), (3), (4), (5), (6)
B. (2), (6) - Skip other steps
C. (5), (2), (1), (6), (4) - Skip step 3
D. (5), (3), (2), (1), (6), (4)
E. (1), (6), (4) - Skip other steps
Close
Session ID: 517697
119
Responses
X
136
2022 Exam 2 #12
Transcribed Image Text:Using a new model lizard with a wild-type strain and genome sequence, you identify a single mutant (the first mutant strain in this organism) that is defective in scale generation. Transgenesis and CRISPR-Cas9 genome editing are possible in this organism. What order of steps (shown below) will be the best approach to identify the gene mutated in this strain? (1) Sequencing of wild-type and mutant strain genomes (2) Mapping (3) Complementation (4) Transgenic rescue of the mutant phenotype by the wild-type gene (5) Assess dominance (6) CRISPR of the gene in the wild-type strain A. (1), (2), (3), (4), (5), (6) B. (2), (6) - Skip other steps C. (5), (2), (1), (6), (4) - Skip step 3 D. (5), (3), (2), (1), (6), (4) E. (1), (6), (4) - Skip other steps Close Session ID: 517697 119 Responses X 136 2022 Exam 2 #12
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Genetic variation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education