The results of the fluctuation test (Fig. 7.5) were interpreted to mean that different numbers of mutantbacteria preexisted in each of the 11 culture tubes because the mutations arose spontaneously at differenttimes during the growth of each culture. However, another possibility is that the differences in the numberof colonies on the plates are simply due to differencesin the ability of the petri plates to support the growthof colonies. For example, perhaps the selective agentor the nutrients in the media were not evenly distributed in the molten agar poured into the petri dishes.What experiment could you do to determine whetheror not differences in the petri plates were a factor inthe experiment?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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The results of the fluctuation test (Fig. 7.5) were interpreted to mean that different numbers of mutant
bacteria preexisted in each of the 11 culture tubes because the mutations arose spontaneously at different
times during the growth of each culture. However, another possibility is that the differences in the number
of colonies on the plates are simply due to differences
in the ability of the petri plates to support the growth
of colonies. For example, perhaps the selective agent
or the nutrients in the media were not evenly distributed in the molten agar poured into the petri dishes.
What experiment could you do to determine whether
or not differences in the petri plates were a factor in
the experiment?

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