The lower chromatogram has a shorter run time because: D 3000- 2500- 2000- 1500- 1000- 500- 0 3000- 2500 2000 1500 1000 500- 10 min. The upper chromatogram was obtained under constant flow conditions The carrier pressure is increased to maintain constant flow during the temperature program O The carrier flow increases as the oven temperature increases O The upper chromatogram was obtained at a lower isothermal oven temperature Constant pressure GC separation 20 min. Constant flow GC separation

The lower chromatogram has a shorter run time because: D 3000- 2500- 2000- 1500- 1000- 500- 0 3000- 2500 2000 1500 1000 500- 10 min. The upper chromatogram was obtained under constant flow conditions The carrier pressure is increased to maintain constant flow during the temperature program O The carrier flow increases as the oven temperature increases O The upper chromatogram was obtained at a lower isothermal oven temperature Constant pressure GC separation 20 min. Constant flow GC separation

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Scientists determined the %w/w H20 in methanol by GC, using a capillary column coated with a nonpolar stationary phase and a TCD detector. A linear regression gives a relationship between %w/w H20 (x) and Peak Height (y) as follows: y= 89.71 x- 0.3024 Calculate the %w/w H20 in a sample giving a peak height of 4.23. O A. 0.0379 O B. 0.0505 O C. 379.17 O D.0.0472 O E. 0.0438
1 24) The method of internal standard was used to analyze and measure the concentration of chemical X by GC A known mixture of compounds X and Y (internal standard) gave the following chromatographic results: Compound X Y Concentration (M) 6.3 x 10-8 2.0 x 10-7 a) 2.0 x 10-6 M b) 8.4 x 10-7 M c) 6.6 x 10-7 M d) 4.1 x 10-7 M e) 2.9 x 10-7 M Peak Area 395 787 Then, an unknown was analyzed as follows. 3.0 mL of unknown X was treated with 0.100 mL of 1.6 x 10-5 M Y and the mixture diluted to 10.0 mL final volume. Peak areas were 633 (for X) and 520 (for Y). What is the concentration of X in the unknown. (The original 3.00 sample?)
Question 8 Borneol and isoborneol were formed in a reaction. Use the following GC chromatogram of the isoborneol and borneol dissolved in acetone to answer the question: Please note that the question is below the chromatogram. 15000 14000 12000 10000- 8 8000. 6000 0 10 0.5 GC of Bomeal and Isobomeol 0.82 Component Acetone Isoborneol Borneol 1.5 Time (min) 8.06E+04 4.84E+04 Peak Area Boiling Point (deg. C) 1.61E+04 56 210 3 213 2.14 2.45 The numbers above each peak represent the time that it took the peak to come out of the column and be detected. What is the term given to this value?
Bradford assay was used to determine bovine serum albumin (BSA) concentrations. The method works by binding Coomassie Brilliant Blue dye to protein standards and unknown, leading to a shift in the absorbance maximum of the dye. A stock solution of BSA is available (10 mg/ml) from which the protein standards were prepared. Bradford reagent, 290 ul, was pipetted into a transparent 96-well microplate. 10 ul of the protein dilution was added followed by mixing in the wells. After 5 min of incubation at room temperature, the plate was read in spectrum mode with the absorbance spectrometer of the microplate reader. The spectrum of the dye not bound to protein was also determined. Create a standard curve or regression equation that related the protein concentration and absorbance. An unknown BSA sample was prepared the same way as the standard and the OD was 0.35. determine the original concentration of the protein solution. The measurements can be seen below. 0.0625 Protein Concentration…
A sample of enzyme involved in alcohol metabolism, extracted and purified extensively from yeast cultures, was loaded onto a gel filtration column for molecular weight approximation. A set of protein standards with known molecular weight was eluted separately through the same column and suitable buffer following the elution of blue dextran for void volume determination. The following table summarizes the data obtained from the experiment: Elution flow rate 1 mL/min Sample collector setting Continuous; Sequential Container change rate 1 tube/min Sample/Standard eluted Test tube No/s. MW (Da) Blue dextran 4 2,000,000 Bovine thyroglobulin 11 669,000 Horse spleen apoferritin 14 443,000 Beta-amylase from sweet potato 21 200,000 Bovine serum albumin 27 66,000 Alcohol metabolism enzyme 19 ? 1. Process the given raw data in a tabular format of properly identified data points ready for calibration curve plotting. 2. Construct the calibration curve using MS Excel for use in MW determination.…
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A silica TLC plate is developed in a mixture of solvent system/mobile phase. The solvent travels 30 mm up the plate Analyte #1 travels 9 mm up the plate Analyte #2 travels 16 mm up the plate Which analyte is more polar, and what is its Rf? Analyte #1, Rf = 0.53 Analyte #2, Rf = 0.53 Analyte #2, Rf = 0.3 Analyte #1, Rf = 0.3
I have set up my spreadsheet and was able to reproduce the result with a pKa of 4.64. TRUE OR FALSE
uestion 5 of 24 Identify the components in the single-beam spectrophotometer schematic. Then, match the spectrophotometer component with its description. Each component will be used once when labeling the diagram and once when matching to the description. P Po 0. Answer Bank detector light source monochromator sample cuvet It holds the sample and has a defined pathlength. It provides electromagnetic radiation in the ultraviolet (UV), visible, or infrared (IR) region of the spectrum. It is typically a grating, prism, or filter that selects a narrow wavelength band of radiation and passes it to the sample. It is typically a photomultiplier tube that generates an electric signal when struck by photons.
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You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is 2.0*10^(-4) M Determine and justify whether the components are part of a displacement system, and if so, provide their free and bound concentrations. Assume that the number of binding sites is the same for all components.
A chromatographic column was found to have the following constants: A= 0.01 cm, B= 0.30 cmis and C=0.015s. What are the optimum flow rate? A none of the choices Bp-15 cmis p-45 cm/s D u-3.6 cmis E-26 cm/s