A silica TLC plate is developed in a mixture of solvent system/mobile phase. The solvent travels 30 mm up the plate Analyte #1 travels 9 mm up the plate Analyte #2 travels 16 mm up the plate Which analyte is more polar, and what is its Rf? Analyte #1, Rf = 0.53 Analyte #2, Rf = 0.53 Analyte #2, Rf = 0.3 Analyte #1, Rf = 0.3

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A silica TLC plate is developed in a mixture of solvent system/mobile phase.

- The solvent travels 30 mm up the plate.
- Analyte #1 travels 9 mm up the plate.
- Analyte #2 travels 16 mm up the plate.

**Question**: Which analyte is more polar, and what is its Rf?

**Options**:
- ○ Analyte #1, Rf = 0.53
- ○ Analyte #2, Rf = 0.53
- ○ Analyte #2, Rf = 0.3
- ○ Analyte #1, Rf = 0.3

**Explanation**: 
The retention factor (Rf) is calculated by dividing the distance traveled by the analyte by the distance traveled by the solvent. Analyte #1 is more polar if it travels less distance since polar compounds tend to have lower Rf values on a polar stationary phase like silica gel. 

For Analyte #1:
- Rf = 9 mm / 30 mm = 0.3

For Analyte #2:
- Rf = 16 mm / 30 mm = 0.53

Thus, Analyte #1 is more polar, with an Rf of 0.3.
Transcribed Image Text:A silica TLC plate is developed in a mixture of solvent system/mobile phase. - The solvent travels 30 mm up the plate. - Analyte #1 travels 9 mm up the plate. - Analyte #2 travels 16 mm up the plate. **Question**: Which analyte is more polar, and what is its Rf? **Options**: - ○ Analyte #1, Rf = 0.53 - ○ Analyte #2, Rf = 0.53 - ○ Analyte #2, Rf = 0.3 - ○ Analyte #1, Rf = 0.3 **Explanation**: The retention factor (Rf) is calculated by dividing the distance traveled by the analyte by the distance traveled by the solvent. Analyte #1 is more polar if it travels less distance since polar compounds tend to have lower Rf values on a polar stationary phase like silica gel. For Analyte #1: - Rf = 9 mm / 30 mm = 0.3 For Analyte #2: - Rf = 16 mm / 30 mm = 0.53 Thus, Analyte #1 is more polar, with an Rf of 0.3.
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