1. You wish to determine the %concentration of a stock solution of brilliant blue FCF (MW=792.8grams/mole; extinction coefficient2s=97000M'cm''). You make a 1:50 dilution of stock and then you take lul from your 1:50 dilution and dilute up to 1ml in a 1cm diameter cuvette. You obtain an A625=0.1. What is your total dilution factor? What is the % concentration of the dye in the original stock? 625

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1. You wish to determine the %concentration of a stock solution of
brilliant blue FCF (MW=792.8grams/mole; extinction
coefficient25=97000M'cm'). You make a 1:50 dilution of stock and
then you take lul from your 1:50 dilution and dilute up to 1ml in a
1cm diameter cuvette. You obtain an A625=0.1. What is your total
dilution factor? What is the % concentration of the dye in the original
stock?
Transcribed Image Text:1. You wish to determine the %concentration of a stock solution of brilliant blue FCF (MW=792.8grams/mole; extinction coefficient25=97000M'cm'). You make a 1:50 dilution of stock and then you take lul from your 1:50 dilution and dilute up to 1ml in a 1cm diameter cuvette. You obtain an A625=0.1. What is your total dilution factor? What is the % concentration of the dye in the original stock?
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