1. You wish to determine the %concentration of a stock solution of brilliant blue FCF (MW=792.8grams/mole; extinction coefficient2s=97000M'cm''). You make a 1:50 dilution of stock and then you take lul from your 1:50 dilution and dilute up to 1ml in a 1cm diameter cuvette. You obtain an A625=0.1. What is your total dilution factor? What is the % concentration of the dye in the original stock? 625

1. You wish to determine the %concentration of a stock solution of brilliant blue FCF (MW=792.8grams/mole; extinction coefficient2s=97000M'cm''). You make a 1:50 dilution of stock and then you take lul from your 1:50 dilution and dilute up to 1ml in a 1cm diameter cuvette. You obtain an A625=0.1. What is your total dilution factor? What is the % concentration of the dye in the original stock? 625

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: Solution )100 mL 0.02M potassium permanganate potassium permanganate Mw = 158.03 g mol-1 potassium…

A: Use the molarity formula to calculate moles and the mass of a potassium permanganate.

Q: Measure the mass of 0.400g of Co(NO3)2 + 6H2O and dissolve it in 15mL of distilled or deionized…

A: Limiting reagent is that reacts completely in the reaction. Number of moles of…

Q: A solution was made by dissolving 9.0g of hydrated aluminium sulphate Al2(SO)3 in 250cm3 of…

A: Molarity is number of moles of solute dissolved per litre of solution.

Q: Consider the reaction below: Pb(NO3)2(aq) + 2NH4I(aq) --> PbI2(s) + 2NH4NO3 25 g of 5.0% NH4I (MW…

Q: A). Exactly 4.57g of BaCl2.2H₂O are dissolved in sufficient water to give 250 mL of solution.…

A: Formality of a solution is the measure of its concentration. It is defined as the number of grams of…

Q: How many milliliters of 0.10 M Pb(NO 3 )2 should be used to make ni(en)(h2o)4so4 10% mole excess?

A: This question must have a known value of the amount of Pb(SO4)2 produced.... So see the complete…

Q: A 20.330 g sample of aqueous waste leaving a fertilizer manufacturer contains ammonia. The sample is…

A: Titration is a volumetric analysis in which the unknown concentration of the sample is measured by…

Q: During the standardization of NaOH , an air bubble was present in the tip of the buret when making…

A: Standardisation happens by following V1*S1 = V2 * S2 formula, where V1 = volume of standard…

Q: Craw McGraw-Hill Education Campus Saved Ex. 1b - Mass/volume percent concentration - calculate using…

A: Volume of solution = 0.350 L Mass of KCl = 13.0 g We have to find m/v percent of solution.

Q: Sample 1 2 3 4 Sample Volume, mL 50.00 49.50 25.00 50.00 Ba(OH)2 Volume, mL 43.17 42.68 21.47 43.33…

A: In order to find the mean w/v percentage of acetic acid in the sample. we have to find the w/v…

Q: I CottizO, ,+ L6O2-76ocOz+ Lott2+362.5 KJ what are the molors masses You You might need ? H2O? +20?

A: 1 mole of glucose and 6 mole of oxygen gives 6 mole of carbon dioxide and 6 mole of water. As shown…

Q: The scatter plot below shows the density of ethylene glycol solutions vs. the mass % of those…

A: Use the trendline equation to find out the mass percent of ethylene glycol in a solution for a given…

Q: For the chemical reaction HCN(aq) + KOH(aq) — H,O(l) + KCN(aq) write the net ionic equation,…

Q: (i) (ii) (iii) (iv) OMe Me но н 110 Br SnMe3 OSiMe3 CHO OH NaNH, NH3 (1) Phl, Pd(PPH3)4 THF, reflux…

A: The aim is to draw the product by using wittig, Simmon Smith, coupling reaction etc. it show C-C or…

Q: What is the molarity of a solution with 4.15 grams of solute and a volume of 3,490.0 mL? The solute…

A: Mass of solute = 4.15 g Molar mass of solute = 311.8 g/mol Volume of solution = 3490.0 mL = 3.490 L…

Q: Your first task is to prepare 10.0 mL of a 1.50 x 10-6 M solution of a small protein (FW: 3600…

Q: 5. Compute the molarity of a solution that contains 5.62 g of Na₂CO3 in enough water to make 750.0…

A: The objective of this question is to calculate the molarity of a solution. Molarity is a measure of…

Q: Excess (NH4)2SO4 ( NH 4 ) 2 SO 4 was added to a 70.0 mL 70.0 mL solution containing BaCl2 BaCl 2…

A: Answer:

Q: Write the empirical formula for the hydrated KAl(SO4)2, based on moles of anhydrous KSI(SO4)2=…

Q: Statement of the Problem, A solution of hydrochloric acid was prepared by diluting 25.00 mL of the…

A: Given, Mass percentage of HCl = 37.2% Molar mass of HCl = 36.45 g/mole Density = 1.19 g/mL…

Q: 5. You make a calibration curve using dilute concentrations of dye and determine the equation of the…

Q: A solution of 48.9% H₂SO4 by mass has a density of 1.39 g/cm at 293 K. A 24.0 cm³ sample of this…

Q: 4. A mixture containing only BaO (FW 153.33) and CaO (FW 56.08) weighs 2.00g. The oxides are…

Q: Suppose you have a soil that is made up of 10% organic matter with CEC = 200 cmol/kg, 40% kaolinite…

A: Given: A soil with- 10% organic matter with CEC = 200 cmol/kg 40% kaolinite with CEC = 10 cmol/kg…

Q: a dilution is needed to prepare a standard of 10 mg Zn/L from 0.5g Zn/L. To carry out the dilution…

A: a) Concentration of stock solution, C1 = 0.5 g Zn/l Concentration of standard solution, C2 = 10 mg…

Q: A reaction profile (not to scale!) for the reaction C₂H5Br + OH-C₂H5OH + Br is shown below: E (kJ)…

A: We are given a reaction C2H5Br + OH– → C2H5OH + Br– and a graph for the potential energy of this…

Q: A student synthesized 3.214g of Ni(NH3),Cl2 from 4.023g of NiCl2•6H2O. He dissolved 0.81g of…

Question

1. You wish to determine the %concentration of a stock solution of
brilliant blue FCF (MW=792.8grams/mole; extinction
coefficient25=97000M'cm'). You make a 1:50 dilution of stock and
then you take lul from your 1:50 dilution and dilute up to 1ml in a
1cm diameter cuvette. You obtain an A625=0.1. What is your total
dilution factor? What is the % concentration of the dye in the original
stock?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Calculation of initial dilution factor

VIEW

Calculating the total dilution factor

VIEW

Calculating the concentration of final diluted solution using Beer's law

VIEW

Calculating the molar concentration of stock solution

VIEW

Writing the molarity expression for the stock solution

VIEW

Step 7

VIEW

Step 8

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 8 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

A 25mL volumetric pipet is used to deliver a sample of the stock solution marked “0.6000M X2SO4” into a 100mL volumetric flask. Distilled water is added to the flask until it is about 3/4thfull, the solution is mixed well, then more water is added to fill it up to the calibration mark, then it is mixed again. Calculate the concentration of X+ion in the dilute solution made above.
Mganga is doing a routine analysis in a copper plating factory. He is regularly making up solutions of verypure CuSO4.5H2O. He suspect that a batch of a reagent used to make up a stock solution of CuSO4.5H2Ois impure and it may contains some CuCl2. He asked you to determine the percentage impurity as follows:Dissolve1.500g of a mixture and make up to 1.00L mark.150mL of this solution was treated with 20.00mLof 0.100M AgNO3 to remove 98.6% of chloride as AgCl precipitate.
6. A stock solution containing 25% w/v of chlorhexidine is used to prepare an antiseptic such that when the antiseptic is diluted 1 in 25, a 1 in 500 wash solution is obtained which is ready for use by the patient. What volume (ml) of the stock solution is required to prepare 5 litres of the antiseptic to fulfil a bulk manufacturing order?
4
1)A bottle of concentrated aqueous sulfuric acid, labeled 95.00 wt% H2SO4, has a concentration of 16.5 M. Calculate the density of 95.00 wt% H2SO4. 2)Ascorbic acid in 5 vitamin C tablets was measured by a titration against (100 ± 0.002 M) NaOH solution. It required 10.00 ± 0.05 mL NaOH solution to reach the equivalent point. What is the average quantity of ascorbic acid per vitamin tablet?
Please don't provide handwritten solution...
The chemical method of analysis in determination of the blood alcohol content (%BAC) is: K2Cr2O7 + H2SO4 + C2H5OH → Cr2(SO4)3 + K2SO4 + CH3COOH + H2O What are the stoichiometric coefficients for the reaction above? (please enter the coefficients without spaces and with a coma between the numbers, e.g. 1,2,3,4) 2,8,3,2,2,3,11 During a Breathalyzer Test it was determined that 1.75mg of K2Cr2O7 was consumed by the above reaction. Calculate the number of milligrams of ethanol in the test sample. 0.411 Calculate the Blood Alcohol Content in this sample (%BAC).
Following the monograph procedure, a 724-mg of aspirin (MW=180 g/mol) dissolved in 18.5 ml of cold neutralized alcohol. This solution was then initially titrated with 0.101 N sodium hydroxide solution, then later neutralized with 0.104 sulfuric acid. What is the percentage purity of the sample? 1. What is the milliequivalent weight consumed by the acidic titrant? a. 1.5392 g-meq b. 2.0907 g-meq c. 1.4948 g-meq d. 2.1528 g-meq 2. What is the milliequivalent weight consumed by the basic titrant? a. 5.8656 g-meq b. 1.5392 g-meq c. 5.6964 g-meq d. 1.4948 g-meq 3. What is the difference of milliequivalent weight consumed in the reaction? a. -4.1572 g-meq b. 4.3708 g-meq c. 0.5515 g-meq d. 4.1572 g-meq