The following two sequences represent the same gene from two different species: Assume that all changes in the third position are synonymous and all in the first and second positions are nonsynonymous. a) How many synonymous sites (positions) are there in the gene?  b) How many nonsynonymous sites (positions) are there in the gene?  c) How many synonymous substitutions are there between species?

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The following two sequences represent the same gene from two different species: Assume that all changes in the third position are synonymous and all in the first and second positions are nonsynonymous.

a) How many synonymous sites (positions) are there in the gene? 

b) How many nonsynonymous sites (positions) are there in the gene? 

c) How many synonymous substitutions are there between species? 

d) How many nonsynonymous substitutions are there between species? 

e) What is the dN/dS ratio? 

f) What does the dN/dS ratio tell you about the evolution of this sequence? 

Finally, It has been hypothesized that population size affects genome size. What is the proposed relationship? How can it be explained? 

Arg Ala
Ser
Gly
Thr
Arg
Thr Pro Ser
Arg
Arg
Thr Val Leu Leu
CGG GCA TCA GGG ACT CGA ACG CCC TCA CGG CGG ACC GTT CTC CTT
CGA GCC TCG GGA ACC CCA ACG CCC TCA GGG CGC TCC GTC CTG CTT
Arg
Ala
Ser
Gly
Thr
Pro
Thr
Pro Ser
Gly
Arg
Ser Val Leu Leu
Transcribed Image Text:Arg Ala Ser Gly Thr Arg Thr Pro Ser Arg Arg Thr Val Leu Leu CGG GCA TCA GGG ACT CGA ACG CCC TCA CGG CGG ACC GTT CTC CTT CGA GCC TCG GGA ACC CCA ACG CCC TCA GGG CGC TCC GTC CTG CTT Arg Ala Ser Gly Thr Pro Thr Pro Ser Gly Arg Ser Val Leu Leu
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