The following fictitious double-stranded bacterial DNA sequence codes for a fictitious prot Both strands are shown; the top strand reads 5' to 3' left to right, while the bottom strand re 5' to 3' right to left. Transcription begins with and includes the red underlined A/T (top strand/bottom strand) base pair. This is a bacterial sequence, so there are no introns. 5'GTGTCCGTATGATATTGTGAGATGTTATATCCCGCCGTCAACACCATAAAACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC 3' CACAGGCATACTATAACACTCTACAATATAGGGCGGCAGTTGTGGTATTTTGTCCTATTAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG 3' 5'

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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The following fictitious double-stranded bacterial DNA sequence codes for a fictitious protein.
Both strands are shown; the top strand reads 5' to 3' left to right, while the bottom strand reads
5' to 3' right to left. Transcription begins with and includes the red underlined A/T (top
strand/bottom strand) base pair. This is a bacterial sequence, so there are no introns.
5'GTGTCCGTATGATATTGTGAGATGTTATATCCCGCCGTCAACACCATAAAACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC
3′
3' CACAGGCATACTATAACACTCTACAATATAGGGCGGCAGTTGTGGTATTTTGTCCTAT TAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG 5′
a) Which strand is used as a template for transcription, the top or the bottom?
b) What are the first 15 nucleotides of the resulting mRNA? Indicate the 5' and 3' ends.
c) What is the translation of the first 15 nucleotides of the mRNA?
d) Do the underlined nucleotides TAA encode a stop codon for the protein? Explain.
e) A mutation occurs which results in the insertion of an extra G/C (top strand/bottom strand)
base pair immediately after the G/C base pair shown in blue. What effect will this insertion
mutation have on the mRNA transcript and on the resulting protein?
f) A different mutation results in the substitution of the purple T/A base pair with a G/C base
pair. (The mutation of (e) doesn't happen.) How would this mutation affect the sequence of the
protein that is produced?
Transcribed Image Text:The following fictitious double-stranded bacterial DNA sequence codes for a fictitious protein. Both strands are shown; the top strand reads 5' to 3' left to right, while the bottom strand reads 5' to 3' right to left. Transcription begins with and includes the red underlined A/T (top strand/bottom strand) base pair. This is a bacterial sequence, so there are no introns. 5'GTGTCCGTATGATATTGTGAGATGTTATATCCCGCCGTCAACACCATAAAACAGGATAATCGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC 3′ 3' CACAGGCATACTATAACACTCTACAATATAGGGCGGCAGTTGTGGTATTTTGTCCTAT TAGCGGACGACCCCGTTTCCGCCACTTCCATTTCCACAACGG 5′ a) Which strand is used as a template for transcription, the top or the bottom? b) What are the first 15 nucleotides of the resulting mRNA? Indicate the 5' and 3' ends. c) What is the translation of the first 15 nucleotides of the mRNA? d) Do the underlined nucleotides TAA encode a stop codon for the protein? Explain. e) A mutation occurs which results in the insertion of an extra G/C (top strand/bottom strand) base pair immediately after the G/C base pair shown in blue. What effect will this insertion mutation have on the mRNA transcript and on the resulting protein? f) A different mutation results in the substitution of the purple T/A base pair with a G/C base pair. (The mutation of (e) doesn't happen.) How would this mutation affect the sequence of the protein that is produced?
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Introduction

DNA, a molecule found in all living things, is the carrier of genetic information. It is a long, double-stranded helix made up of nucleotides, which are composed of a sugar, a phosphate group, and a nitrogenous base. The nitrogenous bases in DNA include adenine, thymine, guanine, and cytosine. The sequence of these bases in the DNA molecule determines the genetic code, which provides instructions for the development, growth, and function of all living things. DNA replication is the process by which DNA is duplicated before cell division, ensuring that each daughter cell receives an identical copy of the genetic material. DNA also serves as a template for RNA synthesis during transcription, which in turn directs the synthesis of proteins during translation. DNA plays a fundamental role in the inheritance of traits from one generation to the next.

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