The Enzyme Lactase Experiment Design How does pH affect how lactase drops work to break down lactose into glucose and galactose? What variable will you change in this experiment? This is also called the Independent variable. How will you change it? What variable will you measure in this experiment to determine the effect of the variable you changed? This is also called the Dependent variable. How will you measure it? What variables will you keep the same in this experiment? Check off the items in this list that you would use for this experiment. You may add any items you feel you would need. Skim milk (which contains lactose) Lactase drops Graduated cylinder Eye dropper Ice cubes pH paper Hot plate Mixing spoon Thermometer Vinegar (acidic) Test tubes Glucose test strips Baking soda (basic) Other items you feel you would need to use for this experiment. Describe what you would do in your experiment. For each step, explain why the step is important. Add more steps if required. Step: Why this step is important: If you actually conducted this experiment, what safety issues would you need to identify? If you actually conducted this experiment, what appropriate precautions would you take to ensure that you performed the experiment safely? If you actually conducted this experiment, what results would you expect to see? Why would you expect these results
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
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