The drawing below depicts a restriction map of a segment of a human chromosome. The cut sites shown (1 through 6) are known to be polymorphic and can be present or absent sending upon the genetic makeup of a specific individual's chromosomes. E identifies Eco RI sites and P identifies Pst 1 sites. The line below the map identifies the region of homology between the DNA and a probe used in Southern blot analyses to characterize this region. A. Individual 1 has restriction sites 1-6 (all) on both copies of this chromosome. If DNA from this individual was digested with Pst I, what are the sizes of the fragments that would be detected via Southern blow using the identified probe? B. DNA from individual 1 is digested with both Eco RI and Pst I and then analyzed by Southern blot. What bands are revealed? C. Individual 2 is homozygous for a polymorphism that eliminates site 3. If DNA from this individual was ingested with both Pst I and Eco RI, what are the sizes of the fragments that would be detected via Southern blot?
The drawing below depicts a restriction map of a segment of a human chromosome. The cut sites shown (1 through 6) are known to be polymorphic and can be present or absent sending upon the genetic makeup of a specific individual's chromosomes. E identifies Eco RI sites and P identifies Pst 1 sites. The line below the map identifies the region of homology between the DNA and a probe used in Southern blot analyses to characterize this region.
A. Individual 1 has restriction sites 1-6 (all) on both copies of this chromosome. If DNA from this individual was digested with Pst I, what are the sizes of the fragments that would be detected via Southern blow using the identified probe?
B. DNA from individual 1 is digested with both Eco RI and Pst I and then analyzed by Southern blot. What bands are revealed?
C. Individual 2 is homozygous for a polymorphism that eliminates site 3. If DNA from this individual was ingested with both Pst I and Eco RI, what are the sizes of the fragments that would be detected via Southern blot?
D. Individual 3 has not previously been characterized over this chromosomal locus. The research team digests the DNA with Pst I and Eco RI and generates the following banding pattern. What is the likely genotype of this individual?
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