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The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples   The following results was obtained:       Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown   Abs. BSA   0   0.130   0.350   0.620   0.870   1.120   1.400   1.600 0.635   Abs. BIg   0    0.080   0.230   0.460   0.520   0.410   0.510   0.700        Calculate the concentration of the unknown protein in mg/ml

The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples   The following results was obtained:       Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method Protein (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown   Abs. BSA   0   0.130   0.350   0.620   0.870   1.120   1.400   1.600 0.635   Abs. BIg   0    0.080   0.230   0.460   0.520   0.410   0.510   0.700        Calculate the concentration of the unknown protein in mg/ml

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples

 

The following results was obtained:

 

 

 

Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method

Protein (mg/ml)

0.00

0.125

0.25

0.50

0.75

1.00

1.50

2.00

Unknown

 

Abs. BSA

 

0

 

0.130

 

0.350

 

0.620

 

0.870

 

1.120

 

1.400

 

1.600

0.635

 

Abs. BIg

 

0 

 

0.080

 

0.230

 

0.460

 

0.520

 

0.410

 

0.510

 

0.700

 

  

 

Calculate the concentration of the unknown protein in mg/ml

 

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