One common way to determine phosphorus is by mixing 12-molybdophosphate complex with ascorbie acid to give an intense blue-colored species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A calibration graph was constructed as follow. Deduce the analytical technique used and calculate the concentration of unknown water samples analyzed which demonstrated absorbance reading of 0.269 (sample 1), 0.532 (sample 2) and 0.478 (sample 3). P calibration curve 0.8 0.6 0.4 y = 0.218x + 0.004 R2 = 0.9996 0.2 0.5 1 1.5 2 2.5 3 3.5 Concentration (ppm) Absorbance

One common way to determine phosphorus is by mixing 12-molybdophosphate complex with ascorbie acid to give an intense blue-colored species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A calibration graph was constructed as follow. Deduce the analytical technique used and calculate the concentration of unknown water samples analyzed which demonstrated absorbance reading of 0.269 (sample 1), 0.532 (sample 2) and 0.478 (sample 3). P calibration curve 0.8 0.6 0.4 y = 0.218x + 0.004 R2 = 0.9996 0.2 0.5 1 1.5 2 2.5 3 3.5 Concentration (ppm) Absorbance

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

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One common way to determine phosphorus is by mixing 12-molybdophosphate complex
with ascorbic acid to give an intense blue-colored species called molybdenum blue. The
absorbance of molybdenum blue can be measured at 650 nm. A calibration graph was
constructed as follow. Deduce the analytical technique used and calculate the concentration
of unknown water samples analyzed which demonstrated absorbance reading of 0.269
(sample 1), 0.532 (sample 2) and 0.478 (sample 3).
P calibration curve
0.8
0.6
0.4
y = 0.218x + 0.004
R2 = 0.9996
0.2
0.5
1
1.5
2
2.5
3
3.5
Concentration (ppm)
Absorbance

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