(a) Calculate the absorbance at 430 nm and 600 nm for th following indicator concentrations: 3.0 x10-4 M, 2.0 ×10-4 M. and 1.0 x10-4 M, 0.5 x10-4 M and 0.25 ×10-4 M. (b) Plot absorbance as a function of indicator concentration. (c) Discuss the linearity of the graph of the two absorbance functions.

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Chapter1: Chemical Foundations
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The equilibrium constant for the conjugate acid/base pair is
8.0 x10-5
HIn + H₂O
H3O+ + In. From additional information:
Species
Absorption
Molar absorptivity
Maximum, nm
430 nm
600 nm
HIn
430
8.04×10³
1.23x10³
In
600
0.775×103
6.96x103
(a) Calculate the absorbance at 430 nm and 600 nm for th
following indicator concentrations: 3.0 x10-4 M, 2.0 × 10-4 M.
and 1.0 ×10-4 M, 0.5 ×10-4 M and 0.25 ×10-4 M.
(b) Plot absorbance as a function of indicator
concentration.
(c) Discuss the linearity of the graph of the two absorbance
functions.
Transcribed Image Text:The equilibrium constant for the conjugate acid/base pair is 8.0 x10-5 HIn + H₂O H3O+ + In. From additional information: Species Absorption Molar absorptivity Maximum, nm 430 nm 600 nm HIn 430 8.04×10³ 1.23x10³ In 600 0.775×103 6.96x103 (a) Calculate the absorbance at 430 nm and 600 nm for th following indicator concentrations: 3.0 x10-4 M, 2.0 × 10-4 M. and 1.0 ×10-4 M, 0.5 ×10-4 M and 0.25 ×10-4 M. (b) Plot absorbance as a function of indicator concentration. (c) Discuss the linearity of the graph of the two absorbance functions.
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