The at toin gere was souted from the bacterium Bacilus thuringiersis Chromosome Bacilius thuringiensis Bt toxin gene ard cloned vto E col. E coli plasmid The full-ength t gene and neo gene Bt gene ragments of the gene were gated to the ne gene -Ful-length Bt gene neo Cene Partial Bt genes constructs nee Promoter Restriction site PolyA) mee consensus sequence Expression vector These gene corstructs were inserted into an epresion vector nea Promoter Poly(A) consensus sequence A. tumefaciens Agrobactum umefaciens was transformed with the spresion vector Ti plasmid Bacterial chromosome 00 Recombination Infection Recombination trarsterned the gene constructs along with the promoter and polyAl sequences to the Ti plasmd Tobacco plents were inlected with the recombinant A tuberfaciens, and the Bt gere constructs were transfened to the plant crits

Q: The map of the lac operon is shown below. Consider the following examples that include both haploids…

A: Lac operon is the segment of DNA which includes regulatory gene, structural gene, promoter gene and…

Q: Two bacterial genes are transduced simultaneously. What does this suggest about the location of the…

A: Genetic recombination in bacteria occurs by transformation, conjugation and transduction.

Q: What would be the modifier to add to this HCPCS code?

A: HCPCS Level II codes are a combination of alphabets and numerals assigned to medical services,…

Q: Here is a cell with 2 copies of the lac operon. (assuming there is NO glucose around), figure out if…

A: Lac operon is the segment of DNA which is responsible for controlling the break down of lactose into…

Q: Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an…

A: The objective of the question is to identify the statement that is not true for both LacI and TrpR…

Q: Predict the effect on the activity of the lac operon of a mutation that disrupts the function of: a)…

A: The Catabolic Activator Protein (CAP) is a protein which acts as a positive regulating factor for…

Q: Here is the model for TfR mRNA regulation (see Fig.), for your convenience. Orange protein (IRP or…

A: The transferrin receptor (TfR) is a glycoprotein associated with cell membrane. It plays a major…

Q: What is a consensus sequence? How similar to the consensus sequence would you expect the promoter of…

A: Introduction  An area of DNA upstream of a gene called a promoter, as it relates to genomics, is…

Q: Consider the two-line model of a gene below: A Y X A Y If the promoter is found to the left of the…

A: Transcription of DNA to RNA is a key step that is part of central dogma of life. Life cannot be…

Q: Which of the following is false when the genes of the Lac operon are being tran- scribed in high…

A: Lac operon is a set of genes used by E.coli to metabolize lactose when glucose is not available for…

Q: Consider the two-line model of a gene below: A X A Y B 1 +1 If RNA polymerase is moving to the right…

A: Deoxyribonucleic acid, or DNA, is the genetic material found in humans and the majority of other…

Q: Contrast the characteristics of prokaryotic and eukaryotic genes. What  are the implications for…

A: Prokaryotic genes are typically organized into operons which are functional units that contain…

Q: Describe how one might determine which protein in E. coli are repressed when a culture is shifted…

A: The Escherichia coli is a facultatively anaerobic, rod-shaped, gram-negative, coliform bacterium.…

Q: Which of the following statements about the attempt to express a eukaryotic gene in bacteria is…

A: The regulation of gene expression in eukaryotes occurs at several level, which is far more diverse…

Q: According to the scenario shown, how many segments of DNA (one, two, or three) are removed during…

A: Genetics is the branch of biology which deals with genes, heredity, and genome in the organism.…

Q: a) What is a gene promoter? b) What proteins bind to a promoter? c) What do those proteins do to…

A: a) A gene promoter is a region of DNA located at the beginning of a gene sequence. It serves as a…

Q: Describe the functions of the RAG1 and RAG2 and NHEJ proteins.

A: Deoxyribonucleic acid (DNA) is a long molecule. DNA contains the instructions an organism required…

Q: The regulation of the trp operon genes by the Trp repressor protein is an example of ______ gene…

A: The regulation of the trp operon genes by the Trp repressor protein is an example of  negative gene…

Q: Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse…

A: Wnt4 gene -- Wtn4 name is derived from the first two family members discovered in int1 mouse and…

Q: What is the Use of a promoterless lacZ gene?

A: In prokaryotes, the DNA-binding protein controls the expression and function of genes by controlling…

Q: Nucleosome-free regions: A) are always only associated with genes that are being actively…

A: Introduction :- The organization of genetic material in eukaryotic organism's nucleus is done with…

Q: Which of the following accurately describes the function of HOTAIR? O It is a long non-coding RNA…

A: Option 1 is the correct answer.

Q: Describe what process leads to gene conversion. What are the consequences of gene conversion for…

A: Gene conversion is the process by which one DNA sequence replaces a homologous sequence such that…

Q: If p63 can bind to the same promoter elements as p53, why would it be considered an inhibitor of…

A: Promoters are DNA sequences that function as a kind of "On" switch to start the biological process…

Q: Explain karyopherin exportins? What is it? What is its function? Why is is important for the…

A: Karyopherins, otherwise called importins or exportins, are essentially a family of nuclear transport…

Q: Considering Figure 2-13, if you had a homozygous double mutant m3/m3 m5/m5, would you expect it to…

A: In biology, a mutation is a change in the nucleotide sequence of an organism's genome, virus genome,…

Q: What are Focused core promoters ?

A: Promoters are DNA regions where gene transcription begins. Focused core promoters are one of the two…

Q: Which genes are under the control of the lac promoter?

A: In the bacterial system, set of two or more than two or more than two genes are under the direct…

Q: Based on the partial diploid sketched shown in Figure 3, where cross-hatched rectangles and the red…

A: An operon is defined as the working unit of DNA that are carrying a cluster of genes below the…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.