Task #3: Design primers1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg121 cctctgccgg gaggagcgag gggaccqcag gcgggggcgc atgacctcag gagccgcgcc181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt241 atttctacac ctccgtgtcc cggcccqgcc gcggggagcc ccgcttcatc tcagtgggct301 acgtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc481 agagcgaggc cgttgcqtga ccccqgcccg gggcgcaggt cacgactccc catcccccac541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccgcgggaa) First you'll need to design primers to PCR-amplify amino acids 45-56.i)Record the sequences of the forward and reverse primers, in the 5' to 3' direction. Eachprimer must be 8 nucleotides long (note that normally primers are much longer than this).Show intermediate steps in your primer design process and/or explain how you determinedthe primer sequences.ii) Using the method described in the pre-tutorial material, calculate the Tm for each primer (in°C).

A primer is a short single-stranded nucleotide chain used for the initiation of DNA synthesis. In…

Answered: Task #3: Design primers 1 agagtctcct…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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U UUU UAU 11yr Phe UUC UUA Leu UUG Jle UCU UCC UCA UCG UGU UGC Cys UGA Stop UAG Skop UGG Trp UAC Ser UAA Stop CUU CUC CỦA CUG CAU JHis CAC CGU CGC CGA CGG CCC Leu Pro Arg ССА Delete CCA CG CAA CAG JGIN Gln AUU AUC le AUA AUG Met AGU 1 Ser AGC AGA AGGJArg 1. ACU ACC The ACA ACG AAU M AAC AAA AAG Jlys JAun ATG AAC TAC CTA GGG ACA GAU JAsp GAC GAA GAG JGlu GUU GUC Val G GUA GCU GCC GCA Ala GCG GGU GGC Gly ATG ACC TAG GGA CA GGA GGG GUG G. Compare translation before and after the deletion. What effect might it have on gene function? Asp Daspartic acid lleI isolcucine Thr T threonine Leu L leucine Ser S serine Туr Y tyrosine Glu E glutamic acid Phe F phenylalanine Pro P proline His H histidine Lys K Arg R Gly G glycine lysine Ala A alanine arginine Cys C cysteine Trp W tryptophan Val V valine Gln Q glutamine Met M methionine Asn N asparagine Second Position UGU 1Cy UCU UCC Ser UCA UG UUU Phe UAU UAC Ty UAA Slop UGA Stop UAG Slop UGG Trp UGC UUC U UUA Leu ] low UUG CUU CÚC A Leu CCU CCC Pro…
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