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Answer the following two questions based on the information provided: Kpn3781 restriction enzyme recognizes the following sequence: cuts that sequence in this fashion: 5'-CCGC 3'--GG GG-3' CGCC--- 5' 5' CCGCGG 3' GGCGCC 20. What is the average frequency of that cut site in an organism like Homo sapiens? A) one every 4 nucleotides B) one every 44 nucleotides C) one every 45 nucleotides D) one every 46 nucleotides E) one every 47 nucleotides 21. This enzyme cuts to create: A) blunt ends B) sticky ends with 5' overhangs that can be used in a restriction-ligation reaction C) sticky ends with 3' overhangs that can be used in a restriction-ligation reaction D) sticky ends with 5' overhangs that cannot be used in a restriction-ligation reaction E) sticky ends with 3' overhangs that cannot be used in a restriction-ligation reaction 22. You want to amplify the sequence identified in bold below using PCR, which is part of the genome of E. coli. Ye amplified DNA must contain exactly the fragment in bold and nothing else. What primers could you use from the below? (Spaces have been added for legibility) 5'... GAT GTC GCA GAG TAT CGC GCT GTC TCT... TCC CGC GTG GCG ATC CAG GCC AGC CAC GTT... 3' A) 5' GAT CGC CAC 3' and 5' CGC GCT GTC 3' B) 3' CGC GCT GTC 5' and 3' GAT CGC CAC 5' C) 5' CGC GCT GTC 3' and 5' GTG GCG ATC 3' D) 5' GCA GAG TAT 3' and 5' GCT GGC CTG 3' E) 5' GCA GAG TAT 3' and 5' CAG GCC AGC 3'

Answer the following two questions based on the information provided: Kpn3781 restriction enzyme recognizes the following sequence: cuts that sequence in this fashion: 5'-CCGC 3'--GG GG-3' CGCC--- 5' 5' CCGCGG 3' GGCGCC 20. What is the average frequency of that cut site in an organism like Homo sapiens? A) one every 4 nucleotides B) one every 44 nucleotides C) one every 45 nucleotides D) one every 46 nucleotides E) one every 47 nucleotides 21. This enzyme cuts to create: A) blunt ends B) sticky ends with 5' overhangs that can be used in a restriction-ligation reaction C) sticky ends with 3' overhangs that can be used in a restriction-ligation reaction D) sticky ends with 5' overhangs that cannot be used in a restriction-ligation reaction E) sticky ends with 3' overhangs that cannot be used in a restriction-ligation reaction 22. You want to amplify the sequence identified in bold below using PCR, which is part of the genome of E. coli. Ye amplified DNA must contain exactly the fragment in bold and nothing else. What primers could you use from the below? (Spaces have been added for legibility) 5'... GAT GTC GCA GAG TAT CGC GCT GTC TCT... TCC CGC GTG GCG ATC CAG GCC AGC CAC GTT... 3' A) 5' GAT CGC CAC 3' and 5' CGC GCT GTC 3' B) 3' CGC GCT GTC 5' and 3' GAT CGC CAC 5' C) 5' CGC GCT GTC 3' and 5' GTG GCG ATC 3' D) 5' GCA GAG TAT 3' and 5' GCT GGC CTG 3' E) 5' GCA GAG TAT 3' and 5' CAG GCC AGC 3'

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Answer the following two questions based on the information provided: Kpn3781 restriction enzyme recognizes the following sequence: cuts that sequence in this fashion: 5'-CCGC 3'--GG GG-3' CGCC--- 5' 5' CCGCGG 3' GGCGCC 20. What is the average frequency of that cut site in an organism like Homo sapiens? A) one every 4 nucleotides B) one every 44 nucleotides C) one every 45 nucleotides D) one every 46 nucleotides E) one every 47 nucleotides 21. This enzyme cuts to create: A) blunt ends B) sticky ends with 5' overhangs that can be used in a restriction-ligation reaction C) sticky ends with 3' overhangs that can be used in a restriction-ligation reaction D) sticky ends with 5' overhangs that cannot be used in a restriction-ligation reaction E) sticky ends with 3' overhangs that cannot be used in a restriction-ligation reaction 22. You want to amplify the sequence identified in bold below using PCR, which is part of the genome of E. coli. Ye amplified DNA must contain exactly the fragment in bold and nothing else. What primers could you use from the below? (Spaces have been added for legibility) 5'... GAT GTC GCA GAG TAT CGC GCT GTC TCT... TCC CGC GTG GCG ATC CAG GCC AGC CAC GTT... 3' A) 5' GAT CGC CAC 3' and 5' CGC GCT GTC 3' B) 3' CGC GCT GTC 5' and 3' GAT CGC CAC 5' C) 5' CGC GCT GTC 3' and 5' GTG GCG ATC 3' D) 5' GCA GAG TAT 3' and 5' GCT GGC CTG 3' E) 5' GCA GAG TAT 3' and 5' CAG GCC AGC 3'
Transcribed Image Text:Answer the following two questions based on the information provided: Kpn3781 restriction enzyme recognizes the following sequence: cuts that sequence in this fashion: 5'-CCGC 3'--GG GG-3' CGCC--- 5' 5' CCGCGG 3' GGCGCC 20. What is the average frequency of that cut site in an organism like Homo sapiens? A) one every 4 nucleotides B) one every 44 nucleotides C) one every 45 nucleotides D) one every 46 nucleotides E) one every 47 nucleotides 21. This enzyme cuts to create: A) blunt ends B) sticky ends with 5' overhangs that can be used in a restriction-ligation reaction C) sticky ends with 3' overhangs that can be used in a restriction-ligation reaction D) sticky ends with 5' overhangs that cannot be used in a restriction-ligation reaction E) sticky ends with 3' overhangs that cannot be used in a restriction-ligation reaction 22. You want to amplify the sequence identified in bold below using PCR, which is part of the genome of E. coli. Ye amplified DNA must contain exactly the fragment in bold and nothing else. What primers could you use from the below? (Spaces have been added for legibility) 5'... GAT GTC GCA GAG TAT CGC GCT GTC TCT... TCC CGC GTG GCG ATC CAG GCC AGC CAC GTT... 3' A) 5' GAT CGC CAC 3' and 5' CGC GCT GTC 3' B) 3' CGC GCT GTC 5' and 3' GAT CGC CAC 5' C) 5' CGC GCT GTC 3' and 5' GTG GCG ATC 3' D) 5' GCA GAG TAT 3' and 5' GCT GGC CTG 3' E) 5' GCA GAG TAT 3' and 5' CAG GCC AGC 3'
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