TABLE 13.2 Reactions of the citric acid cycle AG" AG Reaction Enzyme (kJ/mol) (kJ/mol) 1. Acetyl-CoA + oxaloacetate + H2O citrate + CoA-SH + H+ Citrate synthase -32.2 --55 2а. Citrate cis-aconitate + H,O Aconitase +6.3 2b. cis-Aconitate + H20 isocitrate Aconitase 3. Isocitrate + NAD a-ketoglutarate + CO2 + NADH Isocitrate dehydrogenase -11.6 --20 a-Ketoglutarate dehydrogenase complex 4. a-Ketoglutarate + NAD+ + CoA-SH = succinyl-CoA + CO2 + NADH -33.5 -40 5. Succinyl-CoA + P + ADP (GDP) succinate + ATP (GTP) + CoA-SH Succinyl-CoA synthetase -3.8 -0 6. Succinate + FAD (enzyme-bound) fumarate + FADH2 (enzyme-bound) Succinate dehydrogenase 7. Fumarate + H20 2 L-malate Fumarase -3.8 8. L-Malate + NAD+ oxaloacetate + NADH + H+ Malate dehydrogenase +29.7 Net -48.0 115 Note: AG°' value for reaction 3 was calculated from the E' values for a-ketoglutarate/isocitrate (-0.38 V) and NAD/NADH (-0.32 V).

TABLE 13.2 Reactions of the citric acid cycle AG" AG Reaction Enzyme (kJ/mol) (kJ/mol) 1. Acetyl-CoA + oxaloacetate + H2O citrate + CoA-SH + H+ Citrate synthase -32.2 --55 2а. Citrate cis-aconitate + H,O Aconitase +6.3 2b. cis-Aconitate + H20 isocitrate Aconitase 3. Isocitrate + NAD a-ketoglutarate + CO2 + NADH Isocitrate dehydrogenase -11.6 --20 a-Ketoglutarate dehydrogenase complex 4. a-Ketoglutarate + NAD+ + CoA-SH = succinyl-CoA + CO2 + NADH -33.5 -40 5. Succinyl-CoA + P + ADP (GDP) succinate + ATP (GTP) + CoA-SH Succinyl-CoA synthetase -3.8 -0 6. Succinate + FAD (enzyme-bound) fumarate + FADH2 (enzyme-bound) Succinate dehydrogenase 7. Fumarate + H20 2 L-malate Fumarase -3.8 8. L-Malate + NAD+ oxaloacetate + NADH + H+ Malate dehydrogenase +29.7 Net -48.0 115 Note: AG°' value for reaction 3 was calculated from the E' values for a-ketoglutarate/isocitrate (-0.38 V) and NAD/NADH (-0.32 V).

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter19: The Tricarboxylic Acid Cycle

Section: Chapter Questions

Problem 21P: Complete oxidation of a 16-carbon fatty acid can yield 129 molecules of ATP Study Figure 19.2 and...

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