Suppose an organelle contains 0.03 M KCI and 25 mM NaCl. How many grams of sucrose would you need per liter of solution to isolate intact organelles at 25 degrees Celsius (room temperature)? R is the gas constant = 8.315 J/molk T is the absolute temperature in Kelvins. Answer with 2 decimal places as before.

Suppose an organelle contains 0.03 M KCI and 25 mM NaCl. How many grams of sucrose would you need per liter of solution to isolate intact organelles at 25 degrees Celsius (room temperature)? R is the gas constant = 8.315 J/molk T is the absolute temperature in Kelvins. Answer with 2 decimal places as before.

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter3: The Plasma Membrane And Membrane Potential

Section: Chapter Questions

Problem 1SQE: Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following...

See similar textbooks

Related questions

Q: In the experiment showing osmosis through a semipermeable membrane, 3 corn syrup mixtures are used.…

A: Osmosis is the movement of water from its region of higher concentration to its region of low…

Q: The average time it takes for a molecule to diffuse adistance of x cm is given byt = x2/2D where t…

A: Diffusion is the physiological process by which molecules (and ions) move from a region of higher…

Q: Indicate which of the following provides an example of diffusion by answering yes or no. A crystal…

A: Diffusion is a phenomenon in which the molecules move from a region of higher concentration to the…

Q: o get an idea of the different sizes of various cellular components, do the following calculations:…

A: Protein is a macronutrient that is essential to building muscle mass. It is found throughout the…

Q: A researcher is isolating proteins from a cell via different techniques. They find that when the…

A: Establishing protein expression is important in the study of cells, because proteins often carry out…

Q: The tonicity of blood plasma is 0.9% NaCl (equivalent to 99.1% water and 0.9% solutes.) What is the…

A: Tonicity, unlike osmotic pressure, is only affected by solutes that cannot pass the membrane, as…

Q: Organelle mixture 1.09 1.11 Centrifuge 1.15 2 - 1.19 1.22 1.25 Which fraction would you take to…

A: Sucrose density gradient centrifugation is a technique to separate subcellular organelles and for…

Q: Suppose the concentration of glucose inside a cell is 0.1 mM and the cell is suspended in a glucose…

A: The movement of a molecule ion along or against a concentration gradient involves a change in free…

Q: spherical, single-celled organism which needs oxygen to survive. a. Assume oxygen needs to diffuse…

A: The time that the thermal neutron spends diffusing through the reactor materials before it is…

Q: Use the percent method to estimate the size of a human cheek epithelial cell. You will need to use…

A: At 1000x magnification you will be able to see 0.180mm, or 180 microns.

Q: Did urea (molecular weight 60.07) diffuse through the 20 MWCO membrane? Select one: Yes, but very…

A: Dialysis is a separate procedure that helps to move the solute across the semipermeable membrane…

Q: a) Which of the two molecules that are the same size will passively diffuse across a cell membrane…

A: A) water and carbon dioxide can passively move across membrane because of its small size and water…

Q: Table 4: Diffusion times for carbon dioxide as a function of cell size. Cell Diameter (mm) Cell…

A: Cell diameter Diffusion time sec .01 .05 0.1 5 1 500 3 4500 5 12500 10 50000 15 112500…

Q: An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane…

A: Glucose is a monosaccharide that is it cannot be hydrolyzed further. The movement of solvent…

Q: The shape of a yeast cell can be approximated by a sphere.(a) Calculate the volume of each cell…

A: Step 1 Yeasts are unicellular and uninucleate. In case of rapid growth, an incipient or temporary,…

Concept explainers

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.

Question

Suppose an organelle contains 0.03 M KCl and 25 mM NaCl.
How many grams of sucrose would you need per liter of solution to
isolate intact organelles at 25 degrees Celsius (room temperature)?
R is the gas constant = 8.315 J/molk
T is the absolute temperature in Kelvins.
Answer with 2 decimal places as before.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: Objective

VIEW

Step 2: Explanation

VIEW

Solution

VIEW

Step by step

Solved in 3 steps with 17 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following sets of data: a. Given [ Ca2+ ]0=1mM,[ Ca2+ ]i=100nM, find Eca2+ b. Given [ Cl- ]0=110mM,[ Cl- ]i=100mM, find Ecl
Calculate the number of cells in the body of an average 68-kg (150-lb) adult. (This will only be accurate to about 1 part in 10 but should give you an idea how scientists estimate this commonly quoted number.) Assume all cells are spheres 20 m in diameter. The volume of a sphere can be determined by the equation v=4/33. (Hint: We know that about two thirds of the water in the body is intracellular and the density of cells is nearly 1 g/mL. The proportion of the mass made up of water is about 60%.)
Which of the following methods of transport is being used to transfer the substance into the cell in the accompanying graph? a. diffusion down a concentration gradient b. osmosis c. facilitated diffusion d. active transport e. vesicular transport f. It is impossible to tell with the information provided.
Using the answer code on the right, indicate which membrane component is responsible for the function in question: 1. channel formation____________________________________ (a) lipid bilayer 2. barrier to passage of water- __________________ (b) proteins soluble substances ____________________________________ (c) carbohydrates 3. receptor sites 4. membrane fluidity 5. recognition of self 6. membrane-bound enzymes 7. structural boundary 8. carriers
Why is it advantageous for the cell membrane to be fluid in nature?
Visit this link (http://openstaxcollege.org/l/diffusion) to see diffusion and how it is propelled by the kinetic energy of molecules in solution. How does temperature affect diffusion rate, and why?
Discuss why the following affect the rate of diffusion: molecular size, temperature, solution density, and the distance that must be traveled
Why is the relationship between surface area and volume of a cell important in determining cell size limits?
Assume that a membrane permeable to Na+ but not to Cl- separates two solutions. The concentration of sodium chloride on side 1 is higher than on side 2. Which of the following ionic movements would occur? a. Na+ would move until its concentration gradient is dissipated (until the concentration of Na+ on side 2 is the same as the concentration of Na+ on side 1). b. Cl- would move down its concentration gradient from side 1 to side 2. c. A membrane potential, negative on side 1, would develop. d. A membrane potential, positive on side 1, would develop. e. None of the preceding is correct.
One of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?
Figure 3.23 Which categories of amino acid would you expect to find on the surface of a soluble protein, and which would you expect to find in the interior? What distribution of amino acids would you expect to find in a protein embedded in a lipid bilayer?
List the means of unassisted membrane transport.