Organelle mixture 1.09 1.11 Centrifuge 1.15 2 - 1.19 1.22 1.25 Which fraction would you take to obtain mitichondria, and why? O Fraction 2 because mitochondria contain haemoglobin, giving them a reddish colour O Fraction 3 because it is the most dense fraction O It is impossible to decide without further testing O Fraction 1 because it is the least dense fraction O Fraction 2, because mitochondria contain haem, giving them a reddish colour Increasing density of sucrose (g/cm³)

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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The image below shows a density gradient centrifugation carried out to separate a mixture of lysosomes, peroxisomes and mitochondria
Organelle
mixture
60L
1.11
Centrifuge
1.15
2
1.19
1.22
1.25
Which fraction would you take to obtain mitichondria, and why?
O Fraction 2 because mitochondria contain haemoglobin. giving them a reddish colour
O Fraction 3 because it is the most dense fraction
O It is impossible to decide without further testing
O Fraction 1 because it is the least dense fraction
O Fraction 2, because mitochondria contain haem, giving them a reddish colour
Increasing density of
sucrose (g/cm³)
3.
Transcribed Image Text:The image below shows a density gradient centrifugation carried out to separate a mixture of lysosomes, peroxisomes and mitochondria Organelle mixture 60L 1.11 Centrifuge 1.15 2 1.19 1.22 1.25 Which fraction would you take to obtain mitichondria, and why? O Fraction 2 because mitochondria contain haemoglobin. giving them a reddish colour O Fraction 3 because it is the most dense fraction O It is impossible to decide without further testing O Fraction 1 because it is the least dense fraction O Fraction 2, because mitochondria contain haem, giving them a reddish colour Increasing density of sucrose (g/cm³) 3.
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