Suppose the concentration of glucose inside a cell is 0.1 mM and thecell is suspended in a glucose solution of 0.01 mM.(a) What would be the free energy change involved in transporting10-6 mole of glucose from the medium into the cell? AssumeT = 37 °C.(b) What would be the free energy change involved in transporting10-6 mole of glucose from the medium into the cell if the intracel-lular and extracellular concentrations were 1 mM and 10 mM,respectively?(c) If the processes described in parts (a) and (b) were coupled to ATPhydrolysis, how many moles of ATP would have to be hydrolyzed inorder to make each process favorable? (Use the standard free energychange for ATP hydrolysis.)

The movement of a molecule ion along or against a concentration gradient involves a change in free…

Answered: Suppose the concentration of glucose…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The dialysis bag membrane in the figure is permeable to water and to the simple sugars only. The final solute concentration will: • Stay same without change • Reach equilibrium on both sides. • Sucrose concentration will be higher inside the bag. "Environment" 0.01 M sucrose 0.01 M glucose "Cell" • Glucose concentration will be higher inside the bag. 0.03 M sucrose 0.02 M glucose 0,01 M fructose • Fructose concentration will be higher inside the bag.
In the experimental conditions described below, how many molecules of dextrose do you have to add to the extracellular fluid in order to make it iso-osmotic relative to the intracellular fluid? Intracellular fluid: • Number of water molecules = 60 • Number of Dextrose molecules = 8 • Number of Sucrose molecules = 4 Extracellular fluid: • Number of water molecules = 160 • Number of dextrose molecule = ???? • Number of Sucrose molecules = 0 Enter the number of molecules of dextrose in your answer (format: for 7 enter 7 or 7.00, for 7.5 enter 7.5 or 7.50, for 7.3333 enter 7.33 etc.). Your Answer:
The enzyme urease increases the rate of urea hydrolysis at pH 8.0 and 20 °C by a factor of 10¹4. Suppose that a given quantity of urease can completely hydrolyze a given quantity of urea in 3 minutes at pH 8.0 and 20 °C. How long would it take for this amount of urea to be hydrolyzed in the absence of urease at the same temperature and pH in sterile conditions? time uncatalyzed = years
In the experimental conditions described below, how many molecules of dextrose do you have to add to the extracellular fluid in order to make it iso-osmotic relative to the intracellular fluid? Intracellular fluid: Number of water molecules = 60 • Number of Dextrose molecules = 7 • Number of Sucrose molecules = 3 ● Extracellular fluid: • Number of water molecules = 160 • Number of dextrose molecule = ???? • Number of Sucrose molecules = 0
You fill a bag made of semipermeable membrane with a .5 mol/litre solution of sugar, seal it and immerse it into a .3mol/litre solution of salt and water. Which description best fits what will happen. 1) The environment is hypertonic to the contents, so the bag will shrink as water leaves the bag. 2)The environment is hypotonic to the contents, so the bag will expand as water enters the bag. 3) The environment is hypertonic to the contents, so the bag will expand as water enters the bag. 4) The environment is hypotonic to the contents, so the bag will shrink as water leaves the bag.
An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane (but with no cell wall) is immersed in a beaker containing an aqueous solution. The outside environment concentration consists of 0.01 M glucose and the inside of the cell has a concentration of 5.0 M glucose. The plasma membrane is permeable to water and monosaccharides, but impermeable to the disaccharides. Complete the following for the image below: Is the glucose going down or against its concentration gradient? Is the movement of the solute in the cell going out of the cell? Down...Yes Down...No Against...Yes Against...No
Shown below are cells (colored) that were recently placed into a beaker containing a clear solution For each scenario, indicate whether movement of the molecule into the cell will occur using facilitated diffusion or active transport. А. B. 125mM 20mM fructose glucose 85MM 35mM fructose glucose OA= facilitated diffusion; B= facilitated diffusion OA= active transport; B= active transport O A= active transport; B= facilitated diffusion A= facilitated diffusion; B= active transport
Estimate the equilibrium potential of a cell at 37 °C if the concentration of K* ions inside the cell is 50 times that on the outside of the cell.
Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25oC) to prevent swelling and lysis? Calculate how much sucrose (342.3 g/mol) would you need to make 500 ml of this extracting solution.
A cell with a K+1 concentration of 0.3 eq/L is placed into a solution with a K+1 concentration of 0.2 eq/L. It is assumed that the K+1 can pass through the cell membrane (the K+1 transport protein is open). Answer the following true or false questions. 1. K+1 will diffuse through the membrane down its concentration gradient due to Kinetic Theory 2. Movement of a solute through the membrane is called dialysis 3. K+1 will move out of the cell

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