Shown is the electrophoretic profile of a pure protein ran at different pH values "H H H H (+) (-) pH 5 pH 6.5 pH 7.4 pH 9.2 What is most likely the reason for the change in the position of th protein band at pH 9.2? O A. deprotonation of internally located basic amino acid residues B. deprotonation of N-terminal amino acid C. deprotonation of an internally located basic amino acid, his D. deprotonation of acidic amino acids, glu and asp OE. deprotonation of cys

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Shown is the electrophoretic profile of a pure protein ran at
different pH values
HH
(+)
(-)
pH 5
pH 6.5
pH 7.4
pH 9.2
What is most likely the reason for the change in the position of the
protein band at pH 9.2?
A. deprotonation of internally located basic amino acid residues
B. deprotonation of N-terminal amino acid
C. deprotonation of an internally located basic amino acid, his
D. deprotonation of acidic amino acids, glu and asp
E. deprotonation of cys
Transcribed Image Text:Shown is the electrophoretic profile of a pure protein ran at different pH values HH (+) (-) pH 5 pH 6.5 pH 7.4 pH 9.2 What is most likely the reason for the change in the position of the protein band at pH 9.2? A. deprotonation of internally located basic amino acid residues B. deprotonation of N-terminal amino acid C. deprotonation of an internally located basic amino acid, his D. deprotonation of acidic amino acids, glu and asp E. deprotonation of cys
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