1 10:16 AM Fri Sep 2<то1. A simplified structure of the peptide Lys-Glu-Ser withjust the ionizable groups in the charged state is shown withthe pKa of each group. Calculate the pl for this peptide.Show your work.pka = 8.5H3N+NH3*pKa 10.02. Draw a dipeptide with a trans linkage and a net charge of -1 at pH 7a. Indicate which six atoms are part of the planar structure of the peptide bond.b. Is the Cis or Trans linkage of a peptide preferred? Why?COO- OHpka=4.23. A mutation that changes an alanine residue in the interior of a protein to a valine is found to leadto a loss of activity. The activity is regained when a second residue is mutated from an isoleucineto a glycine. Why would this mutation restore activity?5. Tetrahydrofuran, THF, a water-soluble ether, has a lower dielectric constant (less polar) thanwater. When THF is added to an aqueous solution of a protein, would you expect the a-helixcontent of the protein to increase or decrease. Why?6. Given the following three amino acid sequences:7. What is SDS and what is its purpose in gel electrophoresis?+:4. The proteases chymotrypsin and trypsin cleave on the C terminal side of Phe and Argrespectively. During purification, one must be more careful with trypsin since it is known to cleaveitself much more readily than chymotrypsin. Why?Peptide # 1: Val-Phe-Val-lle-Val-Pro-Gly-Tyr-Val-Phe-Leu-Val-Pro-Gly-ValPeptide # 2: Met-Ala-Glu-Ala-Glu-Ala-Ala-Met-Lys-Lys-Ala-Ala-Met-LysPeptide # 3: Pro-Gly-Ala-Pro-Gly-Ala-Pro-Gly-Gly-Pro-Gly-Pro-Ala-Gly-Proa. Which peptide is most likely to form an a-helix? Explainpka = 3.5COO-@83%b. Which peptide is most likely to form a ß-sheet? Circle the most likely residues involvedin the turns of the ß-sheet on the peptide your chose above.c. What is the net charge of Peptide #2 at pH 14?(113+

The given peptide contains two amino groups and two carboxylic groups as ionizable groups. The pKa…

1. A simplified structure of the peptide Lys-Glu-Ser with just the ionizable groups in the charged state is shown with the pka of each group. Calculate the pl for this peptide. Show your work. pka = 8.5 H3N+ T NH3* pK₂=10.0 pKa = 3.5 COO- COO- OH pK₂=4.2

1. A simplified structure of the peptide Lys-Glu-Ser with just the ionizable groups in the charged state is shown with the pka of each group. Calculate the pl for this peptide. Show your work. pka = 8.5 H3N+ T NH3* pK₂=10.0 pKa = 3.5 COO- COO- OH pK₂=4.2

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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