Answered: SHOW/IDENTIFY the arrow pushing… | bartleby

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

SHOW/IDENTIFY the arrow pushing mechanism in at least 4 steps.
-20,POCH,
6
-P0,POCH,
1
H
H
phosphoglucose
isomerase (PGI)
CH2OH
H
1
5
н но
2
ОН
H
H
ОН
13
ОН
2
ОН
НО
НО
H
H
Glucose-6-phosphate (G6P)
Fructose-6-phosphate (F6P)

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Help filling in the blanks: Pyridoxal phosphate, PLP, is produced from pyridoxine or vitamin B6. PLP is considered a/an [1C/2C/acyl/amino/carboxyl] carrier. In transaminases, PLP forms a [Schiff base/acetal/phosphoanhydride/condensation] with an amino acid and causes the [a- amino/a-carbon/a-carboxyl] to become [electrophilic/nucleophilic] allowing replacement of the amino group on the amino acid with a ketone. PLP is also used by several other enzymes that work on amino acids because in forming a covalent bond with amino acids it provides for an opportunity for nucleophilic attack and because PLP is conjugated, the cofactor is able to [delocalize/transfer/reduce/stabilize] pi electrons in the substrate and thus [stabilize/delocalize/transfer/reduce] the transition state of the reaction. Production of citrulline occurs in the [cytosol/mitochondrial matrix] while the rest of the urea cycle occurs in the [cytosol/mitochondrial matrix]. Urea is produced in the…
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coo CH,-č-CoA + H,0 Acetyl-CoA CoA CH, но-с-соо coo CH, Citrate čoo Oxaloacetate NADH Çoo CH, сн-соо сн-он NAD CH, Malate но-с-н Coo Isocitrate CoO NAD coo NADH + H CH + co, Fumarate HC Coo CH, CH, a-ketoglutarate FADH, NAD • COA NADH + H • co, FAD Succinate čoo CH, -CH, Succinyl-CoA CH, coo GTP + COA GDP• P. COA In step 4 of this cycle the a-ketoglutarate complex uses the following cofactors or coenzymes which are also used by O A. Zn", Mg**; pyruvate carboxylase В. FAD; dihydrolipoyl transacetylase TPP, COASH, Lipoic Acid; pyruvate carboxylase OP. TPP, COASH, Lipoic Acid; pyruvate dehydrogenase complex O E. biotin; pyruvate dehydrogenase complex
An enzyme that catalyzes a reaction such as the one below is alan: || C-0 || C-0 C=0 С —о — Н I| Н-С—Н H- C H H isomerase O kinase O dehydratase O dehydrogenase O phosphorylase O - I
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C Phosphoglycerate enolase coo O H-C-O-P=O CH₂OH 2-Phosphoglycerate This is a (an) d Tyrosine hydroxylase HO coo NH3 L-Tyrosine This is a (an) 0₂ coo C-O-P-C || CH₂ Phosphoenolpyruvate H₂O + HO HO L-DOPA H₂O coo NH3
NH, This compound is directly involved in: он O=P-0-CH, H но OmP-0- O oxidizing an alcohol in the conversion of sucrose to fructose. O the synthesis of malonyl coenzyme A. reducing a ketone in gluconeogenesis. reducing a C=C double bond in fatty-acid synthesis.
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