Scenario 1. Microbial examination of Food (Aerobic Plate Count) Fifty grams of food sample (vegetable dish) is collected from a cafeteria in Area 2, UP campus using sterile spatula. The sample is transferred into sterile containers and transported to the laboratory immediately after collection.
Scenario 1. Microbial examination of Food (Aerobic Plate Count) Fifty grams of food sample (vegetable dish) is collected from a cafeteria in Area 2, UP campus using sterile spatula. The sample is transferred into sterile containers and transported to the laboratory immediately after collection.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Transcribed Image Text:Scenario 1. Microbial examination of Food (Aerobic Plate Count)
Fifty grams of food sample (vegetable dish) is collected from a cafeteria in Area 2, UP
campus using sterile spatula. The sample is transferred into sterile containers and
transported to the laboratory immediately after collection.
In the laboratory, 450 ml of diluent (phosphate-buffered water) is added to the sample.
The sample is blended in the stomacher to ensure homogenization. Ten mL aliquot of the
sample is transferred to 90 mL phosphate buffer, followed by another two 10-fold
dilutions. The different dilutions are spread plated in triplicate PCA plates and incubated at
37 °C for 24-48 hours. Only the last three dilutions were plated. Colony counts were
recorded as shown in Table below:
Dilution/
Dilution 2
Dilution 3
Dilution 4
Trial
Plate count (cfu)
Trial 1
315
104
17
Trial 2
225
81
15
Trial 3
210
93
20
1. Illustrate the procedure done by the student.
2. The total Aerobic Plate Count is intended to indicate the level of microorganisms in
a product. What is the APC of the food sample?
3. The acceptable limit for aerobic plate count of foods is <10° cfu/ml or g (or less
than 5 log10 cfu/ml or g). Is the Aerobic Plate Count of the vegetable dish within the
acceptable limit? Why or why not? Explain further.
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