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Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 Time (mins) - Group 3(Ampicilin) Group 3(Control) Absorbance(60onm)

Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 Time (mins) - Group 3(Ampicilin) Group 3(Control) Absorbance(60onm)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Procedure A Add 50 ul of Top up to 50ml. - Add Mix 5ml Take 3 ml LB ampicillin to mixture the mixture fresh overnight culture with 45 ml LB to analyse the both broth OD600 Withdraw 3ml mixture to is shaked incubator at 37°c 200rpm The mixture then Measure every 15 mins. Stop until Overnight culture analyse (OD600)record as zero time-point. incubated at (E-coli + LB borth) • 75 mins. Mix overnight culture 5ml Take mixture 3 ml No ampicillin to No ampicilin - B (control) with 45 ml LB broth analyse the OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until incubated shaked - analyse (OD600)record as zero time-point. at incubator at 37°c 200rpm 75 mins. Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3(Ampicillin) Group 3(Control) Measurement of bacterial cell growth in LB Absorbance(6oonm)
Transcribed Image Text:Procedure A Add 50 ul of Top up to 50ml. - Add Mix 5ml Take 3 ml LB ampicillin to mixture the mixture fresh overnight culture with 45 ml LB to analyse the both broth OD600 Withdraw 3ml mixture to is shaked incubator at 37°c 200rpm The mixture then Measure every 15 mins. Stop until Overnight culture analyse (OD600)record as zero time-point. incubated at (E-coli + LB borth) • 75 mins. Mix overnight culture 5ml Take mixture 3 ml No ampicillin to No ampicilin - B (control) with 45 ml LB broth analyse the OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until incubated shaked - analyse (OD600)record as zero time-point. at incubator at 37°c 200rpm 75 mins. Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3(Ampicillin) Group 3(Control) Measurement of bacterial cell growth in LB Absorbance(6oonm)
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