Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Make a flowchart for the procedure
Transcribed Image Text:Procedures:
1. Melt the sterile medium in the 2 test, tunes and cool to 50 degree celsius.
2. Pour into 2 separate petri dishes.
3. While the medium is still in its liquid state, inoculate it with 1ml of the bacterial culture
by pour plate method. Follow aseptic techniques
4. When the agar has hardened using a marketing pen, divide one of the plates into two
equal portions by marking the bottoms of the petri dish.
5. Four small amount of equal portions of sterile water on 2 small beaker. To one dissolve
a small amount streptomycin and to the other a small amount of penicillin.
6. Soak a piece of filter paper disk in streptomycin solution. Aseptically transfer this disk
into one of the divisions on the plate marked earlier and label as streptomycin. Do the
same with the other antitripic solution.
Transcribed Image Text:7. To the other plate mark four equal divisions. Make four quadrants. Label each quadrant
as to the soap you are going to use.
8. Put small amount of sterile water in 4 small beakers. Make four different soap solutions.
9. Soak a filter paper disks in each soap solution. Aseptically transfer each of these disks
into each guardant and label according to the soap name.
10. Incubate the plates at room temperature for 48 hours.
11. After 48 hours measure the zone of inhibition in mm and tabulate your observation
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