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Procedure Mix Take ml Top up to 50ml. Add 50 ul of Sml mixture Add fresh LB ampicillin to the overnight culture with 45 ml LB to mixture analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until Overnight culture incubated analyse (OD600)record as shaked incubator at 37°c 200rpm at (E-coli + LB borth) zero time-point. 75 mins. Mix overnight culture Sml Take 3 ml No ampicillin - mixture analyse to No ampicilin B (control) with 45 ml LB broth the OD600 Withdraw 3ml mixture to The incubated incubator at 37°c 200rpm mixture Measure every 15 mins. Stop until is then shaked -analyse (OD600)record as at zero time-point. 75 mins. Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)

Procedure Mix Take ml Top up to 50ml. Add 50 ul of Sml mixture Add fresh LB ampicillin to the overnight culture with 45 ml LB to mixture analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until Overnight culture incubated analyse (OD600)record as shaked incubator at 37°c 200rpm at (E-coli + LB borth) zero time-point. 75 mins. Mix overnight culture Sml Take 3 ml No ampicillin - mixture analyse to No ampicilin B (control) with 45 ml LB broth the OD600 Withdraw 3ml mixture to The incubated incubator at 37°c 200rpm mixture Measure every 15 mins. Stop until is then shaked -analyse (OD600)record as at zero time-point. 75 mins. Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Based on the graph below, write a discussion in detail.

Procedure Take 3 ml Top up to 50ml. Add 50 ul of Mix Sml ampicillin mixture to the mixture Add fresh LB overnight culture with 45 ml LB to analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. Overnight culture incubated analyse (OD600)record as shaked incubator at 37°c 200rpm at (E-coli + LB borth) zero time-point. Mix overnight culture Sml Take 3 ml No ampicillin - mixture analyse to No ampicilin B (control) with 45 ml LB broth the OD600 The incubated incubator at 37°c 200rpm mixture is then shaked - analyse (OD600)record as Withdraw 3ml mixture to Measure every 15 at mins. Stop until zero time-point. 75 mins. Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)
Transcribed Image Text:Procedure Take 3 ml Top up to 50ml. Add 50 ul of Mix Sml ampicillin mixture to the mixture Add fresh LB overnight culture with 45 ml LB to analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until 75 mins. Overnight culture incubated analyse (OD600)record as shaked incubator at 37°c 200rpm at (E-coli + LB borth) zero time-point. Mix overnight culture Sml Take 3 ml No ampicillin - mixture analyse to No ampicilin B (control) with 45 ml LB broth the OD600 The incubated incubator at 37°c 200rpm mixture is then shaked - analyse (OD600)record as Withdraw 3ml mixture to Measure every 15 at mins. Stop until zero time-point. 75 mins. Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 2 (Ampicillin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)
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