You are given a sputum sample to make serial dilutions on a Friday. The pathologist tells you that the patient is very sick so bacteria should be very concentrated you will need to dilute it (assume that the sample is concentrated at ~1x107 CFUs/mL). But you only have 2 sterile tubes to make dilutions! It is Friday and you want to go home because you are tired and/or want to go have fun with your friends. Since you don’t have time or don’t want to clean and sterilize more tubes to make more dilutions, you decide to make do with the two tubes you have available. How would you dilute your sputum sample to get a countable plate by using only two tubes for dilutions? You have to make sure you get a countable plate. You have to explain your dilutions in detail, this means you need to specify not only dilution factors but the volumes you are using to get to those dilutions.

Q: Possible Unknown Strains: Stapylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus,…

A: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…

Q: Without water for decontamination, you are forced to improvise. What supplies will you grab? Select…

A: Decontamination: Decontamination is the process of removing or neutralizing contaminants from…

Q: The predominance of squamous epithelial cells with few WBCs in a sputum sample would be adequate for…

A: The sputum culture is specific test that checks for bacteria or another type of organism that may be…

Q: According to the CDC Guidelines for Infection Control in Dental Health-Care Settings, all…

A: Centre for Disease Control and Prevention (CDC) is a health department which provides the guidance…

Q: Time to scrub in,” says Dr. Hodges. The appendectomy you are about to observe is your second…

A: The sarcoplasmic reticulum is a membrane-bound structure found within muscle cells that is similar…

Q: Which of the following is FALSE about interpreting the results of normal microbial flora experiment?…

A: Beta hemolysis is complete hemolysis . It is characterized by a clear zone surrounding the colonies.…

Q: Airborne Precautions are needed if  there are fomites on environment surfaces/reusable medical…

A: C. Droplets precautions are also needed

Q: An individual is visiting a family member who was admitted to the intensive care with influenza.…

A: When visiting a family member in the intensive care with influenza, it is important to take steps to…

Q: Please I want the gram negative identification, gram stain reaction, cell shape, cell…

A: An organism belonging to the coliform group can be identified using the IMViC tests, a collection of…

Q: There are many types of chemical agents used in the areas of the hospitals depending where. Do you…

A: Hospital is a place where proper sanitation has to be maintained at all times. Thus, choosing the…

Q: write a short dicsussion based on the graph..

A: In order to write the paragraph, it is important to understand what an absorption spectra means.…

Q: My professor instructed me to make presentation on this topic "Application of FISH for diagnosis of…

A: Title: Application of FISH for Diagnosis of Oral Microbial Diseases.     FISH stands for…

Q: Belinda!” called Kyle, running to catch up to her. Belinda turned around and waited for him.…

A: Gram staining procedure is a testing procedure for bacteria. In this procedure, few chemicals are…

Q: Which of the following should be done first after a needle stick or sharps injury?  Wash the area…

A: First aid is the first and immediate assistance which is given to any person with either a minor or…

Q: Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time…

A: Ampicillin helps in retarding the growth of microorganisms by stopping their growth because it is an…

Q: A fecal transplant can work to cure a patient infected with a very harmful bacteria, such as C.…

A: C.difficile is caused due to following factors :  Long-term usage of antibiotic medications…

Q: Interpret the result. Left side is for NS1 test and the other side for Dengue IgG/IgM test. C C C C…

A: Non structural protein NS1of the dengue virus is secreted into the blood stream of infected patient…

Substance Abuse

Substance abuse refers to the use of illegal drugs in excessive quantities, as well as the misuse of legal substances such as prescription or over-the-counter pharmaceuticals or alcohol for purposes other than those intended. Drug addiction, also known as substance use disorder, is a disease that affects the brain and behavior of a person. It leads to a failure to control the use of any drug or prescription, legal or illegal.