We will be using a variety of media/tests to identify the unknown organisms. In the table below identify what each test can tell us about our unknown organisms. Media or Test What it tells us? Positive Result(s) Negative Result(s) Additional Information (e.g. reagents used, etc.) Blood agar plate Exotoxin Hemolysins produced, are able to destroy red blood cell and hemoglobin -Complete hemolysis, produce clearing around: Beta hemolysis -Incomplete hemolysis, produce greenish discoloration around colonies- Alpha hemolysis No breakdown – Gamma hemolysis Catalase test Citrate slant Gram stain Decarboxylase broth: Lysine Decarboxylase broth: Ornithine DrySlide (Oxidase Test) Hektoen Enteric Agar Mannitol salt agar Milk Agar Motility test MRVP broth: MR MRVP broth: VP Nutrient gelatin tubes Nitrate Reduction Broth Phenol red broth: Glucose Phenol red broth: Lactose Phenol red broth: Mannitol Phenylalanine deaminase broth SIM Starch plate Tributyrin agar plate Tryptone broth Urea agar Detect Bacteria that can produce urease enzyme Pink: Urease production Orange or yellow Urease absence
We will be using a variety of media/tests to identify the unknown organisms. In the table below identify what each test can tell us about our unknown organisms.
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Media or Test |
What it tells us? |
Positive Result(s) |
Negative Result(s) |
Additional Information (e.g. reagents used, etc.) |
|
Blood agar plate |
Exotoxin Hemolysins produced, are able to destroy red blood cell and hemoglobin |
-Complete hemolysis, produce clearing around: Beta hemolysis -Incomplete hemolysis, produce greenish discoloration around colonies- Alpha hemolysis |
No breakdown – Gamma hemolysis |
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Catalase test |
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Citrate slant |
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Decarboxylase broth: Lysine |
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Decarboxylase broth: Ornithine |
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DrySlide (Oxidase Test) |
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Hektoen Enteric Agar |
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Mannitol salt agar |
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Milk Agar |
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Motility test |
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MRVP broth: MR |
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MRVP broth: VP |
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Nutrient gelatin tubes |
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Nitrate Reduction Broth |
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Phenol red broth: Glucose |
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Phenol red broth: Lactose |
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Phenol red broth: Mannitol |
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Phenylalanine deaminase broth |
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SIM |
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Starch plate |
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Tributyrin agar plate |
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Tryptone broth |
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Urea agar |
Detect Bacteria that can produce urease enzyme |
Pink: Urease production |
Orange or yellow Urease absence |
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